新疆药桑椹花青素的提取和测定

选用溶剂浸提法从新疆药桑椹中提取花青素。通过单因素和正交试验优化提取工艺，并采用pH 值示差法测定花青素含量。结果表明：影响花青素得率因素的优先次序分别为：固液比＞提取时间＞提取温度＞溶剂体积比。药桑椹花青素的最佳提取工艺为溶剂体积比(0.1% HCl 溶液:95% 乙醇)40:60、固液比1:20(g/mL)、提取温度50℃、提取时间90min，该条件下花青素得率为0.0305%。pH 值示差法对药桑椹花青素进行定量分析，可信度较高。

Extraction and Determination of Anthocyanins from Morus nigra L. Growing in Xinjiang

Anthocyanins in Morus nigra L. growing in Xinjiang was extracted with ethanol acidified with HCl (95% ethanol plus 0.1% HCl), and determined by pH differential method. The optimum values of extraction parameters including solid-toliquid ratio, length of extraction, extraction temperature and 0.1% HCl-to-95% ethanol ratio were determined by orthogonal array design to be: 1:20, 50 ℃, 90 min and 40:60, respectively, and solid-to-liquid ratio had the most important effect on anthocyanins yield, sequentially followed by length of extraction, extraction temperature and 0.1% HCl-to-95% ethanol ratio. Under the above optimum extraction condions, the anthocyanins yield was 0.0305%. pH differential method presented high reliability in quantifying anthocyanins in Morus nigra L.