河豚鱼骨骼肌蛋白组分析

为了建立一套对河豚骨骼肌总蛋白进行蛋白组分析的技术体系，采用高分辨率的双向电泳技术对河豚骨骼肌总蛋白进行分离，银染后用IMAGEMASTERTM 软件分析2D 凝胶图谱，共检测出112 种以上的蛋白。利用基质辅助质量飞行时间质谱(MALDI TOF MS)对分离较好的33 个蛋白点进行肽质量指纹图谱分析，并用MASCOT 在线软件通过检索NCBI nr，MSDB 及UniProtKB/TrEMBL 等数据库，鉴定其中的21 个蛋白点，包括原肌球蛋白等结构蛋白以及各种与代谢相关的酶、与信号传导和细胞凋亡相关的蛋白等。该体系为动态监测河豚鱼骨骼肌在不同水环境及饲料的不当添加等情况下蛋白表达谱的变化提供了新的方法。

Protein Profile Analysis of Skeletal Muscle of Takifugu rubripes

In order to construct a set of technical systems for protein profile analysis of skeletal muscle of fugu (Takifugu rubripes), total proteins extracted from fugu skeletal muscle were separated using high resolution two dimensional polyacrylamide gel electrophoresis and developed by silver staining in polyacrylamide gel for computer assisted analysis of electrophoresis patterns using software IMAGEMASTERTM. Subsequently, matrix assisted laser desorption ionization time of flight mass spectrometry (Maldi TOF MS ) was applied for identifying the major protein spots on the 2D profile. Of the 112 protein spots detected in a silver-stained 2D polyacrylamide gel, 33 were analyzed using Maldi TOF MS, and 21 were identified using MASCOT against takifugu or zebrafish data in NCBI nr, MSDB, and UniProtKB/TrEMBL, which included structural proteins such as tropomyosins, enzymes or proteins involved in metabolism and signal transduction and apoptosis etc.