| 核桃隔膜多糖的分离纯化及单糖组成分析 |
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| 引用本文： | 高 莉,王 强,帕提古力·玛合木提.核桃隔膜多糖的分离纯化及单糖组成分析[J].食品科学,2010,31(21):182-185. |
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| 作者姓名： | 高 莉 王 强 帕提古力·玛合木提 |
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| 作者单位： | 1.新疆大学生命科学与技术学院 2.新疆大学理化性质测试中心 |
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| 摘 要： | 目的：分离纯化核桃隔膜多糖并分析其单糖组成。方法：核桃隔膜经水提醇沉、冷冻干燥得水溶性多糖，经DEAE-纤维素52离子柱和SephadexG-100凝胶柱分离纯化后得到多糖组分PDJL-A11(diaphragma of juglans regiaL. acid polysaccharide)。运用紫外光谱鉴定纯度，气相色谱分析多糖的单糖组成。结果：核桃隔膜多糖PDJL-A11单糖组成为：阿拉伯糖、果糖、甘露糖、鼠李糖和葡萄糖，其物质的量比为2.11:6.52:8.81:12.59:15.58。结论：建立了核桃隔膜多糖分离纯化的方法，确定了其单糖组成及其物质的量比、可为核桃隔膜药理学的研究提供参考。
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| 关 键 词： | 核桃隔膜 多糖 纯化 气相色谱 单糖组成 |
| 收稿时间： | 2010-02-08 |
Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L. |
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| GAO Li,WANG Qiang,PATIGUL Mahmut.Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.[J].Food Science,2010,31(21):182-185. |
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| Authors: | GAO Li WANG Qiang PATIGUL Mahmut |
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| Affiliation: | 1. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China；
2. Test Center of Physical and Chemical Characteristic, Xinjiang University, Urumqi 830046, China |
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| Abstract: | The purpose of this work was to isolate and purify polysaccharide from diaphragma of Juglans regia L (PDJL) and analyze its monosaccharide composition. A water-soluble crude polysaccharide was obtained by water extraction, ethanol precipitation and freeze-drying. The crude polysaccharide was purified to obtain a fraction of PDJL-A11 on DEAE-cellulose52 column and SephadexG-100 column. The purity of PDJL-A11 was analyzed by UV spectrophotometer. The monosaccharide composition and molecular ratio of PDJL-A11 were analyzed by gas chromatography. The monosaccharide composition of PDJLA11 was composed of arabinose, fructose, mannose, rhamnose and glucose with a molar ratio of 2.11: 6.52: 8.81: 12.59: 15.58. |
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| Keywords: | diaphragma of Juglans regia L polysaccharide purification gas chromatography monosaccharide composition |
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