| HPLC-UV-ESI-MSn法分离鉴定葛根素原料药中4种有关物质 |
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| 引用本文： | 王鹏远,吴彩胜,吴松,张金兰.HPLC-UV-ESI-MSn法分离鉴定葛根素原料药中4种有关物质[J].质谱学报,2009,30(Z1):71-72. |
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| 作者姓名： | 王鹏远 吴彩胜 吴松 张金兰 |
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| 作者单位： | 中国医学科学院&北京协和医学院，药物研究所，北京 100050 |
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| 摘 要： |
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Separation and Characterization of 4 Related Impurities in Puerarin Bulk Drug by HPLC-UV-ESI-MSn |
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| WANGPeng-yuan,WUCai-sheng,WUSong,ZHANGJin-lan.Separation and Characterization of 4 Related Impurities in Puerarin Bulk Drug by HPLC-UV-ESI-MSn[J].Journal of Chinese Mass Spectrometry Society,2009,30(Z1):71-72. |
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| Authors: | WANGPeng-yuan WUCai-sheng WUSong ZHANGJin-lan |
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| Affiliation: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College,
Beijing 100050, China |
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| Abstract: | A rapid, accurate and simple HPLC-UV-ESI-MSn method was developed for the separation and characterization of related impurities in puerarin bulk drug. The molecular structures and mechanism for the fragmentations of the related impurities were proposed by the MSn results obtained, and confirmed by ESI-FTICR-MS which provided accurate molecular formulations at high mass resolution. The molecular structures of the four related impurities are identified as 3’-hydoxypuerarin, genistin-8-C-glucoside, neopuerarin B and neopuerarin A on the basis of fragmentation mechanism and reference data. |
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| Keywords: | puerarin relatedimpurity HPLC-UV-ESI-MSn FTICR-MS 3’-hydoxypuerarin genistin-8-C-glucoside neopuerarinB |
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