骨胶原蛋白的酶解工艺条件

采用胰蛋白酶酶解骨胶原蛋白制备胶原多肽，通过单因素和响应面试验，以水解度为指标，确定胰蛋白酶酶解胶原蛋白的最佳工艺条件。结果表明：最优工艺条件为pH6.91、反应温度60℃、酶添加量200mg/g 胶原蛋白、底物水平0.1/61.5(g/mL)、水解时间为4h；此条件下的胶原蛋白水解度达到29.36%。酶解后胶原蛋白的溶解性及乳化性均显著提高。

Enzymatic Hydrolysis Processing of Collagen

Collagen was used as the material to prepare collagen polypeptide through enzymatic hydrolysis by trypsin. The optimal hydrolysis conditions were explored by single factor and response surface experiments on the basis of hydrolysis degree. Results indicated that the optimal hydrolysis conditions were hydrolysis pH of 6.91, hydrolysis temperature of 60 ℃, enzyme dosage of 200 mg per 1 g collagen, material-liquid ratio of 1: 615 (g/mL), and hydrolysis time of 4 h. Under the optimal hydrolysis conditions, the hydrolysis degree of collagen was 29.36%. The solubility and emulsification capability of hydrolyzed collagen were significantly improved.