高效液相色谱法测定台农6 号菠萝果实中的糖分

采用高效液相色谱法测定台农6 号菠萝果实中糖分含量的方法。以90% 乙醇作为提取液，色谱柱为CHO-820(钙型)糖柱(300mm × 7.8mm)，柱温90℃，流动相为超纯水，流速0.5ml/min，进样体积10μl，用示差折光检测器检测菠萝果实中的糖分。本方法的相对标准偏差为0.59%～0.85%，相关系数在0.9998 以上，加标回收率为98.84%～100.6%。台农6 号菠萝果肉含糖量与成熟季节有关，夏季果(7 月成熟)和秋季果(9 月成熟)含蔗糖最高，其次为葡萄糖和果糖，冬季果葡萄糖含量最高、蔗糖含量最低。

HPLC Determination of Sugars in “Tainong 6” Pineapple

A method using high performance liquid chromatography (HPLC) was presented for the determination of sucrose, glucose and fructose in “Tainong 6” pineapple. The sugars in pineapple was extracted with 90% ethanol, and the determination was performed on CH-820 (Ca) column (300 mm × 7.8 mm, column temperature 90 ℃) with ultrapure water as a mobile phase at the flow rate of 0.5 ml/min using a refractive index detector. There were good linear relationships between HPLC peak area and concentrations of the three sugars with correlation coefficients not lower than 0.9998. The spike recoveries and the RSDs of precision for the three sugars ranged from 98.84% to100.3% and from 0 .59% to 0.85%, respectively. The sugar content in “Tainong 6” pineapple varied among different harvest seasons. The sucrose content in the pineapple harvested in summer (July) and autumn (September) was the highest, followed by the glucose and fructose contents in turn. Whereas the glucose content in the pineapple harvested in winter (November) was the highest, and the sucrose the lowest.