富贵菜功能成份多糖提取纯化工艺优化

目的：研究富贵菜多糖提取纯化工艺，为有效开发利用功能性食品--富贵菜提供方法学基础。方法：通过正交试验，优化提取方案；采用Sevag 法脱蛋白，双氧水和活性碳脱色，蒽酮- 硫酸法测定多糖含量。结果：在温度100℃，固液比1:7(富贵菜:水，g/mL)，提取时间3.5h，提取次数2 次的条件下，所得多糖含量最高。以氯仿:正丁醇的体积比4:1 为脱蛋白剂，加入0.15g/mL 的活性炭溶液，80℃脱色45min，脱色2 次或加入多糖供试液体积70% 的双氧水试液于60℃脱色120min 效果最佳。结论：该工艺条件简单可行，多糖提取率高，稳定性好，可作为富贵菜多糖的提取纯化工艺。

Extraction and Purification of Polysaccharides from Gynura divaricata (L.) DC.

The extraction yield of Gynura divaricata (L.) DC. polysaccharides affected by extraction temperature, time and times and solid/liquid ratio was optimized by single factor and orthogonal array design methods. In addition, the removals of protein by the Sevag method and of color with hydrogen peroxide or activated carbon from the crude polysaccharide extract obtained were investigated. The anthranone-sulphuric acid method was used for polysaccharide determination. Result showed that a maximum polysaccharide yield was achieved through the optimal twice extraction for 3.5 h each time at 100 ℃ at 1:7 solid/ liquid ratio (g/mL). The optimal solvent for the removal of protein was a mixture of chloroform and n-butanol (4:1, V/V). The addition of 0.15 g/mL activated carbon aqueous suspension for twice treatment for 45 min at 80 ℃ or the addition of 70% (V/V) hydrogen peroxide for 120 min treatment at 60 ℃ gave an optimal removal of color. The procedure proved to be simple, feasible, effective and stable so as to be most suitable for the extraction and purification of polysaccharide from Gynura divaricata (L.) DC..