| 鲜奶中产γ-氨基丁酸的乳酸菌株的筛选与鉴定 |
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| 引用本文： | 李远宏,吕凤霞,邹晓葵,李 颖,陆兆新.鲜奶中产γ-氨基丁酸的乳酸菌株的筛选与鉴定[J].食品科学,2010,31(15):198-202. |
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| 作者姓名： | 李远宏 吕凤霞 邹晓葵 李 颖 陆兆新 |
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| 作者单位： | 1. 南京农业大学食品科技学院
2.无锡市宜兴食品药品监督管理局 |
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| 基金项目： | 国家“863”计划项目(2007AA10Z357) |
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| 摘 要： | 从新鲜牛奶中筛选高产γ- 氨基丁酸(GABA)的乳酸菌株fmbl12-4，通过形态特征、生理生化特征和16S rDNA 序列分析，鉴定菌株为乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)。当fmbl12-4 的湿菌体与100mmol/L L- 谷氨酸钠溶液(L-MSG)按1:20(m/V)混合，于30℃、100r/min 振荡反应24h，转化液中GABA 浓度达到82.36mmol/L。在质量浓度为2.5g/100mL L-MSG 的MRS 培养基中培养6d，GABA 质量浓度达到4.68g/L。
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| 关 键 词： | γ-氨基丁酸 乳酸菌 筛选 鉴定 |
| 收稿时间： | 2009-10-28 |
Isolation and Screening of a Gamma-Aminobutyric Acid-producing Lactic Acid Bacterial (LCB) Strain from Fresh Milk |
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| LI Yuan-hong,LU Feng-xia,ZOU Xiao-kui,LI Ying,LU Zhao-xin.Isolation and Screening of a Gamma-Aminobutyric Acid-producing Lactic Acid Bacterial (LCB) Strain from Fresh Milk[J].Food Science,2010,31(15):198-202. |
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| Authors: | LI Yuan-hong LU Feng-xia ZOU Xiao-kui LI Ying LU Zhao-xin |
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| Affiliation: | 1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China；
2. Yixing Food and Drug Administration, Yixing 214206, China |
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| Abstract: | A lactic acid bacterial strain with high γ-aminobutyric acid (γ-GABA)-producing ability, named fmbl12-4, was isolated from fresh milk. Based on the studies of morphological, physiological and biochemical characteristics and 16S rDNA sequence and phylogenic analyses, fmbl12-4 was identified as Lactococcus lactis subsp. lactis. After 24 h shake (100 r/min) incubation of the wet mycelia of fmbl12-4 in a 20-fold volume of 100 mmol/L L-monosodium glutamate solution at 30 ℃, aγ- GABA content of 82.36 mmol/L was obtained. Incubation in a MRS medium containing 25 g/L L-MSG for 6 days gave 4.68 g/L γ -GABA content. |
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| Keywords: | γ-aminobutyric acid lactic acid bacteria isolation and screening identification |
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