| 香菇多糖酶催化酯化技术及其抗病毒活性研究 |
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| 引用本文： | 杨胜利,刘敏,钱俊青.香菇多糖酶催化酯化技术及其抗病毒活性研究[J].生物质化学工程,2009,43(4):29-32. |
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| 作者姓名： | 杨胜利 刘敏 钱俊青 |
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| 作者单位： | 浙江工业大学药学院, 浙江, 杭州, 310014 |
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| 基金项目： | 浙江省科技厅重点项目 |
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| 摘 要： | 以干香菇多糖为原料,在中性脂肪酶催化下,进行香菇多糖和丙酸的酯化反应研究。通过单因素试验得出最优酯化反应条件为:反应时间30 min、加酶量0.05 g、丙酸用量2 mL、多糖用量0.1 g和反应温度35℃,在此优化的条件下,酯化度为12.26,红外光谱证实有酯键生成。该酯化多糖对Hep G2215 细胞分泌的HBeAg 有不同程度的抑制作用,其治疗指数TI大于2。
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| 关 键 词： | 香菇多糖 酯化 催化 |
| 收稿时间： | 2008-11-21 |
Study on Enzyme Catalytic Esterification Technology of Lentinan Polysaccharide and Antivirus Activity of Its Ester |
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| YANG Sheng-li,LIU Min,QIAN Jun-qing.Study on Enzyme Catalytic Esterification Technology of Lentinan Polysaccharide and Antivirus Activity of Its Ester[J].Biomass Chemical Engineering,2009,43(4):29-32. |
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| Authors: | YANG Sheng-li LIU Min QIAN Jun-qing |
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| Affiliation: | The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China |
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| Abstract: | Enzyme catalytic esterification of lentinan polysaccharide was reported in this paper. The process was optimized through analysis of parameters. The reaction temperature, enzyme dosage, propionic acid dosage, polysaccharide dosage and reaction time were 30 min, 0.05 g, 2 mL, 0.1 g and 35℃, respectively. Under the optimum operational conditions, the degree of esterification is 12.26. IR spectra confirmed the formation of ester. Ester of lentinan polysaccharide had a significant inhibition to HBeAg excretion from Hep G2215 cells with a better therapeutic index(TI). |
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| Keywords: | lentinan polysaccharide esterification catalysis |
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