昆仑雪菊遗传毒性研究

目的研究昆仑雪菊的遗传毒性,为其食用安全性提供科学依据。方法试验受试物为昆仑雪菊浸泡浓缩液,每毫升相当于1 g昆仑雪菊干品。Ames试验和体外哺乳类细胞染色体畸变试验分别设昆仑雪菊浸泡浓缩液0.008、0.04、0.2、1、5 mg/皿组和1.25、2.5、5 mg/ml组,直接计数培养基上各菌株的回变菌落数和染色体畸变细胞数。微核试验、精子畸形试验和小鼠精原细胞或精母细胞染色体畸变试验均设昆仑雪菊浸泡浓缩液低、中、高剂量组(5、10、20 g/kg),各试验分别镜检计数每只动物1 000个嗜多染红细胞(PCE)、1 000个完整精子、100个中期分裂相细胞,记录有微核的嗜多染红细胞数量、畸变精子类型和数目、染色体畸变细胞数。结果昆仑雪菊遗传毒性试验结果均为阴性。结论本研究未发现昆仑雪菊有遗传毒性。

Study on genotoxicity of Coreopsis tinctoria Nutt.

Objective To evaluate the genotoxicity of Coreopsis tinctoria(C.tinctoria) Nutt.extract and provide scientific basis for its edible safety. Methods The test subject was a concentrated solution of C.tinctoria Nutt., equivalent to 1 g of dry matter per milliliter. 0.008,0.04,0.2,1 and 5 mg C.tinctoria Nutt.extract groups were set in Ames test,and the number of reversional mutation colony was counted directly in culture medium.C.tinctoria Nutt.extract 1.25,2.5 and 5 mg/ml groups were set in chromosome aberration test of mammalian cells in vitro, chromosome aberration cell number was directly counted. C.tinctoria Nutt.extract 5,0 and 20 g/kg bodyweight groups were set in micronucleus test, and sperm abnormality test and chromosome aberration test of spermatogonial cell or spermatocyte in mice. 1 000 polychromatic erythrocytes (PCE),1 000 complete sperms and 100 metakinesis cells per animal were observed by microscopy,PCE count with micronuclei count, type of malformed sperms and chromosome aberration cell number were recorded. Results The result of genetic toxicity test were negative. Conclusion C.tinctoria Nutt. extract did not cause any measurable genotoxicity.