LC-MS/MS分析血浆中脂肪酸及代谢产物

建立了高效液相色谱-串联质谱（LC-MS/MS）同时测定血浆中脂肪酸花生四烯酸（AA）及其代谢产物13-羟基十八碳烯酸（13-HODE）和9-羟基十八碳烯酸（9-HODE）的方法。采用Strata-X固相萃取柱净化，BEH C18色谱柱分离，以乙腈 水为流动相，流速0.2 mL/min，等度洗脱；电喷雾离子源负离子模式和多反应监测模式定量。结果表明，3种物质在0.5~50 μg/L范围内线性关系良好，相关系数为0.994 2~0.996 0；低、中、高3个加标水平的平均回收率为97.42%~101.46%；日内相对标准偏差为2.72%~6.11%，日间相对标准偏差为3.87%~6.39%；AA、13-HODE和9-HODE定量限分别为0.5、0.5、1.0 μg/L。该方法简单、灵敏、快速、可靠，可用于血浆中脂肪酸及其代谢产物的检测分析。

Analysis of Fatty Acids and Metabolites in Plasma by LC MS/MS

A method of liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed for the determination of arachidonic acid (AA), 13-hydroxyoctadecadienoic acid (13-HODE) and 9-hydroxyoctadecadienoic acid (9-HODE) in plasma．The plasma sample was extracted using solid phase extraction, then separated on BEH C18 column with equal elution of acetonitrile and water as mobile phase, and the flow rate was 0.2 mL/min. It was detected by electrospray ionization tandem mass spectrometry in the multiple reaction monitoring negative mode. The results show that AA, 13-HODE and 9-HODE have good linear relationship in the concentration of 0.5-50 μg/L. The average recoveries are 97.42%-101.46% at 3 spiked levels. The precisions of intra-day and inter-day are less than 6.4%. The limits of quantification are 0.5, 0.5, 1.0 μg/L for AA, 13-HODE and 9-HODE, respectively. The method is simple, efficient and accurate, which is suitable for determination of fatty acids and metabolites in plasma.