Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatography |
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Authors: | Starkey Jason A Mechref Yehia Byun Chang Kyu Steinmetz Rosemary Fuqua John S Pescovitz Ora H Novotny Milos V |
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Affiliation: | Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA. |
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Abstract: | Capillary electrochromatography using a specialty monolithic matrix was utilized in developing a rapid and highly efficient separation of isoflavones in biological materials. Without a preconcentration technique, it is relatively easy to reach ppm-ppb concentrations of these compounds in soy-based foods and verify them structurally using a photodiode array detector. With on-column preconcentration, we were able to measure low-ppb levels in human serum. Using blood samples from human volunteers, whose diet was supplemented by a soy-based product, the method has been validated for high-throughput screening of isoflavones in clinical studies. |
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