Influence of linoleic acid,pH and HRT on anaerobic microbial populations and metabolic shifts in ASBRs during dark hydrogen fermentation of lignocellulosic sugars |
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Authors: | Subba Rao Chaganti Brahmaiah Pendyala Jerald A Lalman Sathyanarayanan S Veeravalli Daniel D Heath |
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Affiliation: | 1. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Ave., Windsor, ON, Canada N9B 3P4;2. Department of Civil and Environmental Engineering, Center for Engineering Innovation, University of Windsor, 401 Sunset Ave., Windsor, ON, Canada N9B 3P4;3. Department of Biological Sciences, University of Windsor, 401 Sunset Ave., Windsor, ON, Canada N9B 3P4 |
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Abstract: | In this study, controlling an anaerobic microbial community to increase the hydrogen (H2) yield during the degradation of lignocelluosic sugars was accomplished by adding linoleic acid (LA) at low pH and reducing the hydraulic retention time (HRT) of an anaerobic sequencing batch reactor (ASBR). At pH 5.5 and a 1.7 d HRT, the maximum H2 yield for LA treated cultures fed glucose or xylose reached 2.89 ± 0.18 mol mol−1 and 1.94 ± 0.17 mol mol−1, respectively. The major soluble metabolites at pH 5.5 with a 1.7 day HRT differed between the control and LA treated cultures. A metabolic shift toward H2 production resulted in increased hydrogenase activity in both the xylose (13%) and glucose (34%) fed LA treated cultures relative to the controls. In addition, the Clostridia population and the H2 yield were elevated in cultures treated with LA. A flux balance analysis for the LA treated cultures showed a reduction in homoacetogenic activity which was associated with reducing the Bacteriodes levels from 12% to 5% in the glucose fed cultures and 16% to 10% in the xylose fed cultures. Strategies for controlling the homoacetogens and optimal hydrogen production from glucose and xylose are proposed. |
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Keywords: | Anaerobic sequencing batch reactor Hydrogen Glucose Xylose Metabolic flux analysis Microbial diversity |
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