Effect of acid,heat and combined acid-heat pretreatments of anaerobic sludge on hydrogen production by anaerobic mixed cultures |
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| Authors: | Thitirut Assawamongkholsiri Alissara Reungsang Sakchai Pattra |
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| Affiliation: | 1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand;2. Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand;3. Program of Public Health, Department of Science and Technology, Faculty of Arts and Science, Chaiyaphum Rajabhat University, Chaiyaphum 36000, Thailand |
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| Abstract: | Activated sludge (AS) from wastewater treatment plant of brewery industry was used as substrate for hydrogen production by anaerobic mixed cultures in batch fermentation process. The AS (10% TS) was pretreated by acid, heat and combined acid and heat. Combined acid- heat treatment (0.5% (w/v) HCl, 110 °C, 60 min) gave the highest soluble COD (sCOD) of 1785.6 ± 27.1 mg/L with the highest soluble protein and carbohydrate of 8.1 ± 0.1 and 38.5 ± 0.8 mg/L, respectively. After the pretreatment, the pretreated sludge was used to produce hydrogen by heat treated upflow anaerobic sludge blanket (UASB) granules. A maximum hydrogen production potential of 481 mL H2/L was achieved from the AS pretreated with acid (0.5% (w/v) HCl) for 6 h. |
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| Keywords: | Bio-hydrogen Activated sludge Heat Acid |
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