| Bacillus pumilus CN8菌株降解猪血Hb酶的分离纯化及酶学性质研究 |
| |
| 引用本文： | 李浩丽,马美湖,陈文成.Bacillus pumilus CN8菌株降解猪血Hb酶的分离纯化及酶学性质研究[J].食品工业科技,2010(5). |
| |
| 作者姓名： | 李浩丽 马美湖 陈文成 |
| |
| 作者单位： | 华中农业大学食品科技学院,湖北武汉,430070 |
| |
| 基金项目： | 国家十一五科技支撑项目(2006BAD05A16) |
| |
| 摘 要： | Bacillus pumilus CN8菌株的发酵液,经离心分离得到粗酶液,再经硫酸铵盐析、透析,DEAE-Cellulose-52离子交换层析等步骤获得电泳纯的中性蛋白酶。得到比活达686.66U/mg的酶蛋白,纯化倍数为13.2,回收率为35.0%。SDS-PAGE测得其分子量大约为97000Da。该酶的最适作用pH为7.4,最适反应温度为42℃,在pH7.0~8.5范围内较稳定,在30~45℃比较稳定。经酶学性质测定,K+、Mg2+对酶活力具有保护作用,甘油对酶活具有抑制作用。
|
| 关 键 词： | 猪血 血红蛋白 短小芽孢杆菌 分离纯化 酶学特性 |
Study on the isolation,purification and zymetology properties of porcine hemoglobin enzyme degraded by Bacillus pumilus CN8 |
| |
| LI Hao-li,MA Mei-hu,CHEN Wen-cheng.Study on the isolation,purification and zymetology properties of porcine hemoglobin enzyme degraded by Bacillus pumilus CN8[J].Science and Technology of Food Industry,2010(5). |
| |
| Authors: | LI Hao-li MA Mei-hu CHEN Wen-cheng |
| |
| Affiliation: | LI Hao-li,MA Mei-hu,CHEN Wen-cheng(College of Food Science , Technology,Huazhong Agricultural University,Wuhan 430070,China) |
| |
| Abstract: | The Bacillus pumilus CN8 protease was purified from liquor to homogeneity by ultrafiltration,ammonium sulfate precipitation,dialysis and DEAE-Cellulose-52 gel filtration. After that the neutral protease(Bacillus pumilus CN8 protease)was collected which was electrophoresis pure. The specific activity of the enzyme was 686.66U/mg. Purification times and recovery rate of the enzyme were 13.2 and 35.0% respectively. The molecular weight of the purified enzyme was estimated to be 97000Da. The optimum pH was 7.4 ... |
| |
| Keywords: | porcine blood hemoglobin Bacillus pumilus purification zymetology properties |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
