| 脱氧雪腐镰刀菌烯醇酶联免疫检测方法的建立 |
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| 引用本文： | 邓舜洲,游淑珠,许杨.脱氧雪腐镰刀菌烯醇酶联免疫检测方法的建立[J].食品科技,2006,31(8):222-224. |
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| 作者姓名： | 邓舜洲 游淑珠 许杨 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室,南昌,330047 |
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| 摘 要： | 应用抗脱氧雪腐镰刀菌烯醇单克隆抗体12D1建立了竞争间接ELISA方法,用于检测小麦、玉米中的脱氧雪腐镰刀菌烯醇(DON),最低检出限为20ng/mL,检测范围为20￣460ng/mL。用蒸馏水提取掺合DON(250￣4000ng/g)的小麦样品,回收率为82.1%￣96.6%,变异系数为0.6%￣7.1%。
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 检测 |
| 文章编号： | 1005-9989(2006)08-0222-03 |
| 修稿时间： | 2005年1月5日 |
Enzyme linked immunosorbent assay for the detection of Deoxynivalenol |
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| DENG Shun-zhou,YOU Shu-zhu,XU Yang.Enzyme linked immunosorbent assay for the detection of Deoxynivalenol[J].Food Science and Technology,2006,31(8):222-224. |
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| Authors: | DENG Shun-zhou YOU Shu-zhu XU Yang |
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| Abstract: | A competitive indirect ELISA with anti-DON McAb for detecting deoxynivalenol in wheat and corn was developed. The detection limits was 20ng/mL and the linear range of the inhibition curve was 20~460ng/mL. In the detection of the samples of wheat and corn mixed with DON(250~4000ng/g), extracted with water, the recovery rate of DON in the samples ranged from 82.1% to 96.6%, and the coefficients of variation was from 0.6% to 7.1%. |
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| Keywords: | ELISA |
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