不同体系下白腐菌对染料的脱色研究

研究了土豆液体、稻草液体和稻草固体3种培养体系下白腐菌脱色染料的特性。结果表明,含稻草秸秆的培养体系均能较好促进菌株生长、木质素酶系分泌和染料脱色。稻草液体培养体系下生物量在第12天高达3.1g/L;稻草固体培养体系下白腐菌主要分泌漆酶,其次还有锰过氧化物酶和木素过氧化物酶,其中漆酶活力在第5天高达428U/mL,是稻草液体培养体系中漆酶活力的2.9倍;稻草液体和固体培养体系下,菌株分别在第8天和第5天对染料脱色高达90.85%和92.78%,远超过土豆液体培养体系。

Study on the Decolorization of Dye by White Rot Fungus in Different Culture Systems

The characteristics of decoloring dye by white rot fungus in three culture systems(potato liquid culture,straw liquid and straw solid culture)were studied.The results showed that the growth,excretion of ligninase and decolorization of dye could be significantly accelerated in straw liquid and solid cultures.The obtained biomass could be reached 3.1g/L in straw liquid culture on 12th day.The white rot fungus could excrete a mass of Lac and a little of Mnp and Lip.The Lac activity could be achieved 428 U/mL on 5th day,which was 2.9 times in straw solid culture than that in straw liquid culture.The decolorization percentage could be achieved 90.85% and 92.78% respectively in the straw liquid and straw solid culture,which was much more than that in potato liquid culture.