HPLC-ESI-Q-TOF MS/MS分析肉苁蓉拟雌激素活性部位入血成分

为深入研究肉苁蓉(Cistanche deserticola Ma.)活性部位体内作用的物质基础,应用高效液相色谱-四极杆飞行时间质谱(HPLC-Q-TOF MS)技术对肉苁蓉拟雌激素活性部位入血成分进行分析,并与空白血清、肉苁蓉拟雌激素活性部位和对照品之间的色谱图和质谱碎片信息进行对比,共鉴别出13个化学成分,其中包括6个药材原型:京尼平苷酸、红景天苷、肉苁蓉苷A、松果菊苷、毛蕊花糖苷,肉苁蓉苷D;7个代谢成分:3,4-二羟基苯乙醇苷、甲基化毛蕊花糖苷、乙酰化肉苁蓉苷G、3,4-二羟基苯乙醇、乙酰基管花苷B、肉苁蓉苷B葡萄糖醛酸代谢物、甲基化3,4-二羟基苯乙醇苷。该方法可为进一步研究肉苁蓉药理活性和作用机理提供理论支持。

Analysis of Absorbed Components in Rat Plasma after Oral Administration of Quasi Estrogen Fraction of Cistanche by HPLC-ESI-Q-TOF MS/MS

To analyze and identify the constituents in rat plasma after oral administration of the active fraction of cistanche, the components in rat plasma were detected by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (HPLC-Q-TOF MS). Structures of the components were elucidated by comparing their retention times and MS/MS spectra with those of reference compounds and reported data in the literatures. 13 chemical compositions are identified, including 6 prototype components, such as Geniposidic acid, Salidroside, Cistanoside A, Echinacoside, Acteoside, Cistanoside D, 7 metabolites components, such as 3,4-Dihydroxyphenylethanol glycosides, methylation acteoside, acetylation Cistanoside G, 3,4-Dihydroxyphenylethanol, acetyl tubuloside B, Cistanoside B glucuronide metabolite and methylation-3,4 Dihydroxyphenylethanol. The results can provide essential data for illuminate the therapeutic basis of quasi estrogen regulation activity of cistanche.