以低浓度异甘草素为基质的MALDI-TOF MS用于小分子的检测研究

以低浓度异甘草素(ISL,0.05 mmol/L)为基质,采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对精氨酸、甘露醇和苏氨酸-亮氨酸二肽等小分子物质进行分析检测。实验结果表明,以低浓度异甘草素作为MALDI-TOF MS分析小分子物质的有机基质,所获得的斑点均匀性和重复性、耐盐能力、分辨率等均显著优于常用基质2,5-二羟基苯甲酸(DHB)、2,4,6-三羟基苯乙酮(THAP)。本实验可为有机基质在小分子分析中的应用开辟新的窗口。

Low Concentration of Isoliquirigenin as a Matrix for the Analysis of Small Molecules by MALDI-TOF MS

Small molecules, including arginine, mannitol and dipeptide were analyzed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) using a low concentration of isoliquirigenin (ISL, 0.05 mmol/L) as a matrix. In comparison with 2,5-dihydroxybenzoic acid (DHB) and 2,4,6-trihydroxyacetophenone (THAP), ISL exhibited outstanding matrix properties as follows: 1) higher homogeneity of crystallization thus allowing automatic data acquisition; 2) better spectral quality in terms of resolution and signal to noise ratio (S/N); 3) better salt tolerance. The study has an important role in organic compounds as matrix for analyzing small molecules, and can open up a new prospect.