| 树脂对普洱茶多糖的纯化与分离 |
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| 引用本文： | 杨新河,黄建安,刘仲华,毛清黎.树脂对普洱茶多糖的纯化与分离[J].食品科学,2015,36(2):19-24. |
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| 作者姓名： | 杨新河 黄建安 刘仲华 毛清黎 |
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| 作者单位： | 1.湖北工程学院生命科学技术学院，湖北 孝感 432000；
2.国家植物功能成分利用工程技术研究中心，湖南 长沙 410128 |
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| 基金项目： | 国家自然科学基金面上项目(31370692;31370691);湖北省自然科学基金面上项目(2014CFB573) |
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| 摘 要： | 研究10 种树脂对普洱茶多糖脱色和蛋白质去除的效果及DEAE-52纤维素离子交换树脂对普洱茶多糖的分离效果。结果表明，D101树脂适合于对普洱茶多糖同时脱色和蛋白质去除，当普洱茶多糖溶液体积为50 mL时，在pH 4、温度50 ℃、料液质量浓度3.8 mg/mL、树脂用量11 mL的条件下，普洱茶多糖的脱色率为82.33%、蛋白质去除率为70.89%。DEAE-52纤维素离子交换树脂分离经D101树脂处理的普洱茶多糖能得到6 个不同多糖级分。
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| 关 键 词： | 树脂 普洱茶 多糖 纯化 分离 |
Purification and Separation of Pu-Erh Tea Polysaccharide by Resin |
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| YANG Xinhe;HUANG Jian’an;LIU Zhonghua;MAO Qingli.Purification and Separation of Pu-Erh Tea Polysaccharide by Resin[J].Food Science,2015,36(2):19-24. |
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| Authors: | YANG Xinhe;HUANG Jian’an;LIU Zhonghua;MAO Qingli |
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| Affiliation: | 1. School of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China;
2. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China |
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| Abstract: | Among ten types of macroporous resins, D101 resin was selected as the best for the decolorization and
deproteinization of Pu-Erh tea polysaccharides. When 50 mL of the sample at a concentration of 3.8 mg/mL, pH 4 was
adsorbed by 11 mL of D101 resin 50 ℃, the decolorization rate was 82.33% and the removal rate of protein was 70.89%.
Six polysaccharide fractions were obtained after subsequent DEAE-52 cellulose column chromatography. |
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| Keywords: | resin Pu-Erh tea polysaccharide purification separation |
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