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| 响应面法优化蛇足石杉中石杉碱甲的提取工艺 | |
| 引用本文： | 杨婷,武芸,张泽,武玉莲,郑小江,胡世坤,毛鑫. 响应面法优化蛇足石杉中石杉碱甲的提取工艺[J]. 食品科学, 2015, 36(2): 57-62. DOI: 10.7506/spkx1002-6630-201502011 |
| 作者姓名： | 杨婷 武芸 张泽 武玉莲 郑小江 胡世坤 毛鑫 |
| 作者单位： | 1.湖北民族学院 生物资源保护与利用湖北省重点实验室，湖北 恩施 445000；2.湖北民族学院生物科学与技术学院，湖北 恩施 445000；3.湖北民族学院附属民大医院，湖北 恩施 445000；4.四川大学生命科学学院，四川 成都 610064 |
| 基金项目： | 湖北省教育厅科学技术研究项目(D20141903);湖北省自然科学基金项目(2014CFB615);生物资源保护与利用湖北省重点实验室开放基金项目(PKLHB1301);恩施州2013科技研究与开发项目(2013002);国家级大学生创新课题项目(201210517030);湖北民族学院大学生创新训练计划项目(2014Z064);湖北民族学院林学一级学科(0907)资助项目 |
| 摘 要： | 利用响应面分析法优化恩施州蛇足石杉中石杉碱甲的提取工艺条件。在以提取温度、提取时间、料液比、酒石酸质量分数为影响因素，以石杉碱甲提取量为指标的单因素试验基础上，根据Box-Behnken原理设计三因素三水平的试验分析法，得到提取石杉碱甲最佳工艺条件：料液比1∶30（g/mL）、酒石酸质量分数1.5%、 提取温度41 ℃。采用高效液相色谱法检测石杉碱甲含量，在最优提取条件下，热水浸提4 h，石杉碱甲提取量为11.192 8 mg/100 g。 |
| 关 键 词： | 蛇足石杉 石杉碱甲 响应面分析法 高效液相色谱 |
Optimization of Extraction Process for Huperzine A from Huperzia serrata |
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| YANG Ting;WU Yun;ZHANG Ze;WU Yulian;ZHENG Xiaojiang;HU Shikun;MAO Xin. Optimization of Extraction Process for Huperzine A from Huperzia serrata[J]. Food Science, 2015, 36(2): 57-62. DOI: 10.7506/spkx1002-6630-201502011 | |
| Authors: | YANG Ting WU Yun ZHANG Ze WU Yulian ZHENG Xiaojiang HU Shikun MAO Xin |
| Affiliation: | 1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Institute for Nationalities,Enshi 445000, China; 2. College of Biological Scientific and Technical, Hubei University for Nationalities,Enshi 445000, China; 3. University Hospital of Hubei University for Nationalities, Enshi 445000, China;4. College of Life Sciences, Sichuan University, Chengdu 610064, China |
| Abstract: | This paper is concerned with the selection and optimization of important extraction parameters as well as their levels for the increased yield of huperzine A from the aboveground parts of Huperzia serrata by combined use of single factor method and response surface methodology (RSM). Solid-to-solvent ratio, solvent concentration and temperature were identified as main variables that affect extraction efficiency, and their levels were optimized using RSM based on a threevariable, three-level Box-Behnken design. When the extraction was performed for 4 h under the optimized conditions: 1.5% tartaric acid as extraction solvent with a solid-to-solvent ratio of 1:30 (g/mL) and an extraction temperature of 41 ℃, the yield of huperzine A was 11.192 8 mg/100 g. |
| Keywords: | Huperzia serrata huperzine A response surface analysis high performance liquid chromatography |
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