气相色谱法检测油脂中低热量短长链甘油三酯操丽丽，操新民，庞 敏，潘丽军，姜绍通*

利用气相色谱-质谱联用技术结合计算机质谱库检索和特征离子，对短长链甘油三酯进行组分鉴定。建立以DB-17 ht熔融石英毛细管柱作为分析柱，气相色谱-氢火焰离子化检测器分析油脂样品中短长链甘油三酯含量的方法。结果表明，在质量浓度0.5～20.0 mg/mL范围内，含量与峰面积呈现良好的线性关系；各组分的保留
时间和峰面积的相对标准偏差分别小于0.05%和2.0%，方法的重复性好。转酯化和普通大豆油加标回收实验表明，平均回收率分别为98.62%和97.59%。该方法可对短长链甘油三酯各组分进行分离，精确度高、重复性好，可用于油脂中短长链甘油三酯的含量分析。

Determination of Short and Long Chain Triglycerides in Edible Oils by Gas Chromatography

The chemical compositions of short and long chain triglycerides (SLCTs) were analyzed by gas chromatographymass
spectrometry (GC-MS) through mass spectral library searching and characteristic ions. A gas chromatography with
flame ionization detection (GC-FID) method was developed for determining the content of SLCTs in edible oil samples
using DB-17 ht fused silica capillary column as the analytical column. The results showed a good linear correlation between
peak area and concentration in the range of 0.5–20.0 mg/mL. The relative standard deviations for retention time and
peak area of each SLCT were less than 0.05% and 2.0%, respectively, suggesting good reproducibility of the developed
method. The average recoveries from spiked samples of transesterified and original soybean oil were 98.62% and 97.59%,
respectively. This method allowed accurate and highly repeatable quantification of SLCTs in edible oils.