| 生物转化法合成2-苯乙醇菌种的诱变选育 |
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| 引用本文： | 梅建凤,闵航. 生物转化法合成2-苯乙醇菌种的诱变选育[J]. 食品与发酵工业, 2007, 33(5): 22-24 |
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| 作者姓名： | 梅建凤 闵航 |
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| 作者单位： | 1. 浙江工业大学药学院,浙江杭州,310032;浙江大学生命科学学院,浙江杭州,310058
2. 浙江大学生命科学学院,浙江杭州,310058 |
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| 摘 要： | 对3株2-苯乙醇转化菌株的产物耐受性进行了比较,确定了1株产物耐受性较强的菌株,经过自然分离纯化和紫外诱变,筛选获得1株转化合成2-苯乙醇浓度有显著提高的菌株BD-25-39,转化合成2-苯乙醇的浓度达到5.40g/L,较原始菌株提高了10.2%。
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| 关 键 词： | 2-苯乙醇 生物转化 酿酒酵母 菌种选育 |
| 修稿时间： | 2006-09-292006-11-14 |
Breeding of Yeast Strain for Production of 2-phenylethanoi by Biotransformation |
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| Mei Jianfeng,Min Hang. Breeding of Yeast Strain for Production of 2-phenylethanoi by Biotransformation[J]. Food and Fermentation Industries, 2007, 33(5): 22-24 |
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| Authors: | Mei Jianfeng Min Hang |
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| Affiliation: | College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China; 2College of Life Science, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | To breed a high-yield strain for production of 2-phenylethanol by biotransformation, a strain designated BD was elected from three yeast strains after testing of the tolerance to 2-phenylethanol, and then routine separation and ultraviolet radiation treatment was carried out on the strain BD successively. As a result, a mutated strain designated BD-25-39 was screened out, gained the yield of 2-phenylethanol at 5.40 g/ L, which had increased by 10.2% over the original strain. |
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| Keywords: | 2-phenylethanol biotransformation Saccharomyces cerevisiae breeding |
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