番茄皮渣中番茄红素和多糖的提取

以番茄皮渣为原料,采用酶辅助双水相法提取番茄红素和多糖。考察溶剂与盐质量分数、混合酶的组成、酶质量分数、pH、酶解温度及酶解时间对有效成分得率的影响,并采用响应面法对工艺条件进行优化。结果表明:当双水相体系组成为31%乙醇-16%K2HPO4,果胶酶-菠萝蛋白酶为1∶1,酶质量分数为2.0%,在pH 6.0,50℃条件下酶解119 min时,番茄红素和多糖提取得率最高,分别为15.69 mg/100 g和77.16 mg/g。响应面法结果显示,酶质量分数对番茄红素和多糖的提取影响最为显著,其提取结果与预测值相符。与传统溶剂法相比,酶辅助双水相提取法溶剂用量少,提取温度低,提取物具有较好的抗氧化活性。

The Extraction of Lycopene and Polysaccharides from Tomato Pomace

Use enzyme-assisted aqueous two-phase extraction(EA-ATPE)of lycopene and polysaccharides from tomato pomace.The effects of solvent and salt dosage,composition and concentration of mixed enzymes,pH,enzymolysis temperature and enzymolysis time on the extraction rate of lycopene and polysaccharides were investigated,and the process conditions were optimized by response surface method.The results showed the extraction rates of lycopene and polysaccharides were 15.69 mg/100 g and 77.16 mg/g under the extraction conditions of 31%ethanol-16%K2 HPO4,mixed enzymes composition of pectinase and bromelain 1∶1,amount of enzyme 2.0%,pH 6.0,enzymolysis temperature 50℃ and enzymolysis time 119 min.The amount of enzyme had the most significant effect on the extraction of lycopene and polysaccharides,and the extraction results were consistent with the predicted values.In addition,compared with solvent extraction methods,enzyme-assisted aqueous two-phase extraction had advantage of solvent,lower extraction temperature,better yields and antioxidant properties.