| Apg-2过表达对BaF3-MIGR1及稳定表达BCR/ABL的BaF3-P210细胞增殖的影响 |
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| 引用本文： | 李春莉,刘钉宾,袁颖,彭智,陶崑,史梦,曹唯希,冯文莉. Apg-2过表达对BaF3-MIGR1及稳定表达BCR/ABL的BaF3-P210细胞增殖的影响[J]. 中国生物制品学杂志, 2010, 23(2) |
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| 作者姓名： | 李春莉 刘钉宾 袁颖 彭智 陶崑 史梦 曹唯希 冯文莉 |
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| 作者单位： | 李春莉,刘钉宾,彭智,陶崑,史梦,曹唯希,冯文莉(重庆医科大学医学检验性临床血液学教研室临床检验诊断学教育部重点实验室,重庆,400016);袁颖(重庆医科大学附一院普外科,重庆,400016) |
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| 基金项目： | 哈尔滨工业大学校科研和教改项目 |
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| 摘 要： | 目的探讨热休克蛋白Apg-2过表达对小鼠pMIGR1空载体感染细胞BaF3-MIGR1和BCR/ABL转化细胞BaF3-P210(简称BP210)增殖的影响。方法构建pIERS2-EGFP-Apg-2真核表达质粒,电穿孔转染BaF3-MIGR1和BP210细胞,经G418筛选稳定表达细胞株,分别用RT-PCR和Western blot鉴定Apg-2的表达。采用Am-Blue法和流式细胞仪(FCM)检测Apg-2过表达对BaF3-MIGR1和BP210细胞增殖的影响,光镜观察细胞形态变化。结果pIERS2-EGFP-Apg-2真核表达质粒构建正确,转染细胞后筛选到稳定过表达Apg-2的BaF3-MIGR1-Apg-2和BP210-Apg-2细胞株,BaF3-MIGR1-Apg-2细胞的增殖速度明显低于BaF3-MIGR1细胞,BP210-Apg-2细胞比BP210细胞增殖加快。光镜下可见Apg-2过表达的两种细胞形态发生变化,瑞氏染色可见细胞核增多。结论Apg-2能够促进BCR/ABL阳性细胞的增殖,可能与慢性粒细胞白血病的发生发展相关。
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| 关 键 词： | Apg-2 慢性粒细胞白血病 BCR/ABL 细胞增殖 |
Effect of Overexpression of Apg-2 on Proliferations of BaF3-MIGR1 Cells and BaF3-P210 Cells Stably Expressing BCR / ABL |
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| LI Chun-li,LIU Ding-bin,YUAN Ying,et al. Effect of Overexpression of Apg-2 on Proliferations of BaF3-MIGR1 Cells and BaF3-P210 Cells Stably Expressing BCR / ABL[J]. Chinese Journal of Bilogicals, 2010, 23(2) |
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| Authors: | LI Chun-li LIU Ding-bin YUAN Ying et al |
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| Affiliation: | LI Chun-li,LIU Ding-bin,YUAN Ying,et al(Department of Clinical Hematology,Key Laboratory of Laboratory Medical Diagnostics of Education Ministry,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the effect of overexpression of heat shock protein Apg-2 on the proliferations of BaF3-MIGR1 cells infected with empty vector pMIGR1 and BaF3-P210(BP210)cells transformed with BCR / ABL. Methods A eukaryotic expression vector pIERS2-EGFP-Apg-2 was constructed and transfected to BaF3-MIGR1 and BP210 cells by eletroporation. The cell strains for stable expression were screened with G418, and the expression of Apg-2 was identified by RT-PCR and Western blot. The effect of overexpressio... |
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| Keywords: | Apg-2 BCR/ABL Apg-2 Chronic myelogeneous leukemia BCR/ABL Cell proliferation |
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