Purification and Identification of Antioxidant Peptides from the Scallop Skirts of Mimachlamys Nobilis |
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| Authors: | Quanfu Wang Yifan Wang Yanhua Hou Yifeng Xu Meihong Yan Haoyuan Li [JZ]Yuanping Liu and Yujin Li |
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| Affiliation: | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China,School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China,School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China,School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China,School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China,School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, Shandong, China,Shandong Provincial Engineering Technology Research Center of Marine Health Food, Rongcheng 264300, Shandong, China and National and Local United Engineering Laboratory of Marine Functional Food Development Shandong,Rongcheng 264300,Shandong,China |
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| Abstract: | The processes of scallop meats can produce a large amount of by-products like scallop skirts, which are rich in nutrients. This work aimed to obtain the information about antioxidant peptides derived from scallop skirts of Mimachlamys nobilis. The hydro-lysates obtained by trypsin exhibited the highest antioxidant activity (75.6%, 79.5% and 76.7% using DPPH, hydroxyl and superoxide anion radical scavenging activity assays, respectively). The antioxidant peptides (MNAP) were separated and purified using UF membranes, GFC processes and RP-HPLC, and the antioxidant activity of MNAP was identified to be 85.7% using DPPH radical scavenging activity assay. The peptides (MNAP) were composed of two peptides with their molecular weights 2 855.6 Da and 3 007.2 Da using MALDI-TOF-MS, respectively. The results suggested that the scallop skirts of Mimachlamys nobilis could be explored as a source of antioxidant peptides. |
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| Keywords: | Mimachlamys nobilis antioxidant peptides purification scallop skirts hydro-lysates |
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