悬浮填料挂膜阶段硝化效果及其FISH检测研究

采用悬浮填料处理生活污水的挂膜启动试验,研究了挂膜启动过程中出水中主要污染物质随时间的变化情况,并对生物膜中硝化细菌进行检测研究。结果表明,在温度为:20～30℃,溶解氧为3.0～4.0 mg/L,MBBR在19 d左右完成挂膜启动,氨氮去除率达到88%,没有发生亚硝酸盐的积累;荧光原位杂交技术分析揭示生物膜中硝化菌随挂膜时间的变化,第15天生物膜中氨氧化菌和亚硝酸盐氧化菌分别占总真细菌数的14%和10%,第27天生物膜中氨氧化菌和亚硝酸盐氧化菌分别占总真细菌数的22%和19%,硝化细菌在挂膜后期有较大的增殖。

Nitrification Performance and FISH Detection in Startup-test of Biofilm Culturing of Suspended Carrier

The startup-test of the aerobic moving bed biofilm reactor for the domestic wastewater treatment is used to study and analyze the changes of the main pollutants in the different periods of time during the startup-test,and the bacteria of nitrifier in the biofilm was examined.The results show that the biofilm can be cultured successfully in the nineteenth day when the temperature is 20 to 30 ℃ and the DO is 3.0～4.0 mg/L,NH4+-N removal efficiency reaches 88%,and there is no any accumulation of the nitrite in the effluent.Fluorescent in situ hybridization analysis indicates the nitrobacteria within the biofilm change as time goes.The ammonia oxidizing bacteria and the nitrite-oxidizing bacteria occupy about 14% and 10% of the total eubacteria number in the 15th day biofilm,respectively,and in the 27th day biofilm,the ammonia oxidizing bacteria and the nitrite-oxidizing bacteria occupy about 22% and 19% of the total eubacteria number,respectively.In the late startup process,the nitrifying bacteria have a greater proliferation.