| 超临界流体色谱法分析天然维生素E |
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| 引用本文： | 蒋崇文,何德文. 超临界流体色谱法分析天然维生素E[J]. 粮食与油脂, 2005, 0(3): 36-37 |
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| 作者姓名： | 蒋崇文 何德文 |
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| 作者单位： | 中南大学化学化工学院,长沙,410038 |
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| 摘 要： | 以超临界CO2作流动相,乙醇作夹带剂,在Waters Spherisorb silica column(3.9×250 mm, 5μm)上用超临界流体色谱法分离和测定维生素E中三种生育酚异构体(α-生育酚、γ-生育酚和δ-生育 酚)含量。实验压力:18 MPa;温度:40℃;CO2流量:750 ml／min(常压);乙醇流量:0.1 ml／min,三 种生育酚在超临界流体色谱中分析时间小于7分钟,RSD小于3％,超临界流体色谱法与液相色谱法测 定结果基本一致。
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| 关 键 词： | 超临界流体色谱 维生素E 超临界CO2 |
| 文章编号： | 1008-9578(2005)03-0036-02 |
| 修稿时间： | 2005-02-16 |
Analysis of Natural Vitamin E by Supercritical Fluid Chromatography |
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| JIANG Chong-wen,He De-wen. Analysis of Natural Vitamin E by Supercritical Fluid Chromatography[J]. Cereals & Oils, 2005, 0(3): 36-37 |
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| Authors: | JIANG Chong-wen He De-wen |
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| Abstract: | Separation and determining of α-,γ- and 8-tocopherol in natural vitamin E with supercritical fluid chromatography (SFC) were achieved with CO2 as mobile phase and modified with etha-nolon the Waters Spherisorb silica column (3.9×250 mm, 5 μm). The operation pressure and temperature were at 18 MPa and 40℃, and the flux of dioxide carbon/ethanol was 750 mlmin-1 (normal pressure)/0.1 mlmin-1. Three tocopherols were eluted under 7 minutes, and the RSD was less 3%. The result of the determination of tocopherols in natural Vitamin E by SFC was in satisfactory agreement with those obtained by HPLC. |
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| Keywords: | supercritical fluid chromatography vitamin E supescritical CO2 |
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