Studying the interactome with the yeast two-hybrid system and mass spectrometry |
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Authors: | Causier Barry |
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Affiliation: | School of Biology, University of Leeds, Leeds LS2 9JT, United Kingdom. b.e.causier@leeds.ac.uk |
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Abstract: | Protein interactions are crucial to the life of a cell. The analysis of such interactions is allowing biologists to determine the function of uncharacterized proteins and the genes that encode them. The yeast two-hybrid system has become one of the most popular and powerful tools to study protein-protein interactions. With the advent of proteomics, the two-hybrid system has found a niche in interactome mapping. However, it is clear that only by combining two-hybrid data with that from complementary approaches such as mass spectrometry (MS) can the interactome be analyzed in full. This review introduces the yeast two-hybrid system to those unfamiliar with the technique, and discusses how it can be used in combination with MS to unravel the network of protein interactions that occur in a cell. |
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Keywords: | interactome mass spectrometry protein–protein interaction proteomics yeast two‐hybrid |
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