含锶磷灰石骨水泥浆体的pH值及其固化体的体外细胞毒性评价

在线测量了自制含锶羟基磷灰石骨水泥(Sr-CPC)浆体在水化过程中的pH值实时变化以及各固化体在培养液中浸泡不同时间后其浸提液的pH值,并利用MTT比色法评价了该水泥固化体的体外细胞毒性.XRD与FTIR分析表明,固化体在SBF中浸泡24h后,水化产物为锶钙羟基磷灰石固溶体,而且该固溶体中含有与骨磷灰石类似的CO₃^2-;各水泥浆体pH值的在线测量表明,不同含锶量水泥浆体的pH基本在6.5-7.8之间变化,接近中性;体外细胞毒性试验表明,不同含锶量水泥固化体的细胞毒性为0或1级,且细胞毒性与各水泥固化体浸提液的浓度、作用时间有一定的关联性.

pH Value Evolution of a Novel Sr-containing Calcium Phosphate Cement Pastes and Cytotoxicities of Their Hardened Bodies

The online pH value evolutions of a novel Sr-containing calcium phosphate cement (Sr-CPC) pastes together with the extracts of their hardened bodies immersed in the culture medium were measured and the cytotoxicities of various hardened bodies were evaluated by MTT assay. Results obtained from the XRD and FTIR analyses show that most of the Sr-CPC has transformed into Sr-containing apatite solid solution after subjected an immersion process in SBF for 24h. CO32- ion can be detected in the final set product, which is similar to the bone-like apatite. The pH values of various cement pastes basically vary in the range of 6.5-7.8. All extracts exhibit no or low cytotoxicity with a score of 0 or 1 grade according to the six-grade criterion in GB/T 16175-1996. A slight relevance exists among the cytotoxicity and concentration of extracts and acting time.