| 微生物制剂MP喷施烟叶后的差异蛋白分析 |
| |
| 引用本文： | 赵敏,汪长国,刘劲,寇明钰,李宁,吴艳,戴亚,夏庆友.微生物制剂MP喷施烟叶后的差异蛋白分析[J].烟草科技,2012(7):72-75. |
| |
| 作者姓名： | 赵敏 汪长国 刘劲 寇明钰 李宁 吴艳 戴亚 夏庆友 |
| |
| 作者单位： | 1. 川渝中烟工业公司技术中心,成都市成龙大道1段56号,610066
2. 西南大学生物技术学院,重庆市北碚区天生路2号,400715 |
| |
| 基金项目： | 川渝中烟博士后立项项目，重庆市博士后科研项目(渝xm201103009)"生物制剂HL提高烟叶品质的机制研究"，国家高新技术研究发展计划(863计划)项目"复合酶耦合细菌发酵改善烟梗可用性研究"，四川省立项项目"功能性添加剂在雪茄型烟叶发酵过程中的应用研究"，中央高校基本科研业务费专项资金项目"the Fundamental Research Funds for the Central Universities" |
| |
| 摘 要： | 为明确微生物制剂MP(芽孢杆菌)喷施烟叶后对其品质的影响,以喷施微生物制剂MP和清水的烟叶作为研究材料,采用蛋白质双向凝胶电泳法分析了烟叶中蛋白质的表达差异。结果显示,在MP制剂诱导烟叶后,有6个蛋白质的表达量发生了显著变化,其中5个蛋白点上调表达,1个蛋白点下调表达。利用基质辅助激光解析串联飞行时间质谱法(MALDI TOF/TOF MS)对这6个差异蛋白进行了分析,结果共鉴定出4个差异蛋白,分别为烟草酸性几丁质酶PR-P、烟草几丁质酶前体、内切几丁质酶A前体和逆渗透蛋白。几丁质酶和逆渗透蛋白与植物的抗病性密切相关,说明在喷施MP制剂后烟叶可能产生抵御外源病菌的应急机制并进而提高其抗病性。
|
| 关 键 词： | 烟叶 MP制剂 双向凝胶电泳 差异蛋白 |
Analysis of Differential Protein in Tobacco Leaves Sprayed with Microbial Agent MP |
| |
| ZHAO Min
,
WANG Changguo
,
LIU Jin
,
KOU Mingyu
,
LI Ning
,
WU Yan
,
DAI Ya
,
XIA Qingyou.Analysis of Differential Protein in Tobacco Leaves Sprayed with Microbial Agent MP[J].Tobacco Science & Technology,2012(7):72-75. |
| |
| Authors: | ZHAO Min WANG Changguo LIU Jin KOU Mingyu LI Ning WU Yan DAI Ya XIA Qingyou |
| |
| Affiliation: | 1.Technology Center,China Tobacco Chuanyu Industrial Co.,Ltd.,Chengdu 610066,China 2.College of Biotechnology,Southwest University,Chongqing 400715,China |
| |
| Abstract: | In order to find out the effects of Microbial agent MP(Bacillus magaterium)spraying on the quality of tobacco leaves,two-dimensional gel electrophoresis(2-DE)was used to analyze the differences of protein expression between tobacco leaves sprayed with MP and water respectively.The results revealed that the expression levels of six proteins changed significantly,among them the expression levels of five protein points increased,and that of the other one decreased after MP treatment.The six differential proteins were identified with matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI TOF/TOF MS),the results showed that four of them were recognized as tobacco acidic chitinase PR-P,tobacco chitinase precursor,endochitinase A precursor and osmotin respectively.Chitinase and osmotin closely related to plant’s disease resistance,it indicated that the emergency system against exogenous bacteria might be induced and by which disease resistance was improved after MP spraying. |
| |
| Keywords: | Tobacco leaf Microbial agent MP Two-dimensional gel electrophoresis Differential protein |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
