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诱导子对烟草植株叶片酶活性及香气物质积累的效应
引用本文:臧晓静,李永宽,赵高岭,杜雷,高明,席宇,朱大恒.诱导子对烟草植株叶片酶活性及香气物质积累的效应[J].烟草科技,2012(4):76-80.
作者姓名:臧晓静  李永宽  赵高岭  杜雷  高明  席宇  朱大恒
作者单位:1. 郑州大学生物工程系,郑州市科学大道100号,450001
2. 河南中烟工业有限责任公司郑州卷烟厂,郑州市陇海东路72号,450004
3. 黄河流域水资源保护局,郑州市城北路东12号,450004
基金项目:河南省烟草专卖局科技攻关项目
摘    要:采用盆栽试验,通过叶面喷施诱导子,考察了诱导子对烟草次生代谢关键酶活性及香气物质积累的影响.结果表明,经诱导子处理过的烟草叶片苯丙氨酸解氨酶、多酚氧化酶和过氧化物酶活性均高于对照,且腺毛分泌物、石油醚提取物、类胡萝卜素、多酚类物质含量也高于对照.综合考虑,100 mg/L浓度的茉莉酸甲酯诱导子和200 mg/L浓度的壳聚糖诱导子处理烟叶较有利于烟草香气成分的生成.

关 键 词:烟草  诱导子  酶活性  香气成分

Inductive Effects of Elicitors on Enzyme Activity and Aroma Component Accumulation in Tobacco Leaves
ZANG Xiaojing , LI Yongkuan , ZHAO Gaoling , DU Lei , GAO Ming , XI Yu , ZHU Daheng.Inductive Effects of Elicitors on Enzyme Activity and Aroma Component Accumulation in Tobacco Leaves[J].Tobacco Science & Technology,2012(4):76-80.
Authors:ZANG Xiaojing  LI Yongkuan  ZHAO Gaoling  DU Lei  GAO Ming  XI Yu  ZHU Daheng
Affiliation:1) 1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China 2.Zhengzhou Cigarette Factory,China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450004,China 3.Yellow River Basin Water Resources Protection Bureau,Zhengzhou 450004,China
Abstract:The influences of elicitors on the activity of key secondary metabolism enzymes and accumulation of aroma components in tobacco leaves were studied with pot experiments by spraying elicitors on leaf surface.The results showed that the enzyme activities of phenylalanine ammonialyase(PAL),polyphenol oxidase(PPO) and peroxidase(POD) in tobacco leaves treated with elicitors were higher than those in the CK,and the contents of glandular trichome exudates,petroleum ether extract,carotenoids and polyphenols were also higher.On the whole,leaf treatment with elicitors of 100 mg/L methyl jasmonate and 200 mg/L chitosan was beneficial to the formation of aroma components in tobacco.
Keywords:Tobacco  Elicitor  Enzyme activity  Aroma component
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