固定化猴头菌细胞的研究

利用海藻酸钠对深层发酵的猴头菌细胞进行固定化培养 ,就包埋剂的最佳浓度、发酵条件及固定化猴头菌细胞在深层发酵中应用的可能性进行了探讨。结果表明 ,最佳海藻酸钠的质量分数为 2 % ,CaCl2 质量分数为 2 % ,固定化猴头菌细胞在发酵培养基内的代谢与游离细胞的代谢基本一致 ,发酵液中氨基酸含量最高可达 80 0mg/L ,多糖含量最高可达 14 0 0mg/L ,固定化细胞可以连续使用 3 2～ 48d ,在工业生产中是可行的

Study on Immobilization of Hericium erience Cell

The fermentation process of Hericium erience immobilized on alginate has been reported in this paper. The optimum concentration of alginate and the optimum fermentative conditions were also studied . The results showed that the optimum concentration of alginate was 2%and CaCl 2 was 2%, the metabolic level of immobilized cell and free cell appear to be similar, the maximum amino acid content in fermented broth was 800?mg/L, polysaccharide content was 1?400?mg/L. Immobilized cell can be continuously used for 32 to 48 days.