Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid |
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Authors: | Sakayu Shimizu Hiroshi Kawashima Kengo Akimoto Yoshifumi Shinmen Hideaki Yamada |
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Affiliation: | (1) Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, 6O6 Kyoto, Japan;(2) Present address: Suntory Ltd., Mishimagun, 618 Osaka, Japan;(3) Present address: Laboratory of Microbial Science, Institute for Fundamental Research, Suntory Ltd., Mishimagun, 618 Osaka, Japan |
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Abstract: | Mycelia of arachidonic acid-producing fungi belonging to the genusMortierella were found to convert an oil containing α-linolenic acid to an oil containing 5,8,11,14,17-cis-eicosapentaenoic acid (EPA). This conversion was observed when they were grown in a medium containing the oil, glucose and
yeast extract at 28 C. On the screening of various oils, linseed oil, in which α-linolenic acid amounts to about 60% of the
total fatty acids, was found to be the most suitable for EPA production. Under the optimal culture conditions, a selected
strain,Mortierella alpina 20-17, converted 5.1% of the α-linolenic acid in the added oil into EPA, the EPA production reaching 1.35 g/l of culture
broth (41.5 mg/g dry mycelia). This value corresponded to 7.1% (by weight) of the total fatty acids in the extracted lipids.
The lipid was also found to be rich in arachidonic acid (12.3%). Other major fatty acids in the lipid were palmitic acid (4.4%),
stearic acid (3.2%), oleic acid (13.5%), linoleic acid (13.7%), α-linolenic acid (38.5%) and γ-linolenic acid (0.9%). |
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