| 花椒中抗AchE活性物质的提取工艺 |
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| 引用本文： | 许静,戴妙妙,魏蒙月,张赟彬,黄润宇,王思佳. 花椒中抗AchE活性物质的提取工艺[J]. 食品工业, 2020, 0(3): 95-99 |
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| 作者姓名： | 许静 戴妙妙 魏蒙月 张赟彬 黄润宇 王思佳 |
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| 作者单位： | 上海应用技术大学香料香精技术与工程学院;上海市质量监督检验技术研究院;上海中侨职业技术学院食品学院;上海中医药大学公共健康学院 |
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| 基金项目： | 上海市晨光计划项目(17CGB26):薄层色谱生物自显影法分离传统香辛料中乙酰碱胆酯酶抑制剂的研究;上海中医药大学预算内项目:中国特产香辛料中抑制阿尔兹海默症活性成分的分离与精制。 |
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| 摘 要： | 采用Ellman比色测定法,以AchE抑制率为考察指标,通过单因素试验研究溶剂种类及体积分数、料液比、提取温度、提取时间等对AchE抑制率的影响,设计正交试验优化花椒中抗AchE活性物质的提取工艺。结果显示,最佳提取工艺为:提取溶剂80%乙醇溶液,提取温度70℃、提取时间120 min、料液比1︰12(g/mL)。在此条件下提取得到的花椒提取物表现出较高抗AchE活性,AchE抑制率为67.42%。
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| 关 键 词： | 乙酰胆碱酯酶 花椒 正交试验 提取工艺 |
Extraction Process of Acetylcholinesterase Inhibitor in Zanthoxylum bungeanum Maxim |
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| XU Jing,DAI Miaomiao,WEI Mengyue,ZHANG Yunbin,HUANG Runyu,WANG Sijia. Extraction Process of Acetylcholinesterase Inhibitor in Zanthoxylum bungeanum Maxim[J]. The Food Industry, 2020, 0(3): 95-99 |
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| Authors: | XU Jing DAI Miaomiao WEI Mengyue ZHANG Yunbin HUANG Runyu WANG Sijia |
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| Affiliation: | (School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 200234;Shanghai Institute of Quality Inspection and Technical Research,Shanghai 201114;School of Food Science,Shanghai Zhongqiao College,Shanghai 201319;Department of Public Health,Shanghai University of Traditional Chinese Medicine,Shanghai 201210) |
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| Abstract: | The AchE inhibition rate was optimized by Ellman colorimetric assay.The effects of solvent type and ethanol concentration,solid-liquid ratio,extraction temperature and extraction time were studied by single factor experiment and orthogonal array design.The results showed the optimal extraction process was as follows:80%ethanol solution,temperature 70℃,extraction time 120 min and the ratio of material to liquid 1︰12(g/mL).Under this condition,the extract of the Zanthoxylum bungeanum Maxim extract showed higher acetylcholinesterase activity resistance.The AchE inhibition rate reached 67.42%. |
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| Keywords: | acetylcholinesterase Zanthoxylum bungeanum Maxim extraction process extraction technology |
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