鱼腥草醇提取物对甲状腺片诱导的甲亢小鼠生理指标的影响研究

目的研究鱼腥草醇提取物对甲状腺片诱导的甲亢小鼠的影响。方法以鱼腥草为原料用无水乙醇进行提取,在应用甲状腺片灌胃并成功建立甲亢模型小鼠方法的基础上,将模型组40只雄性小鼠按每组10只,随机分为阴性对照组(negative control group, NM)及鱼腥草醇提取物低、中、高剂量组(分别记为R1、R2、R3)。另取10只雄性小鼠作为正常对照组(normal control group, NC),连续灌喂30 d。结果与正常组相比,模型组小鼠体重、摄食量、饮水量、肛温以及血清T3、T4、FT3、FT4、TSH水平均有显著差异(P0.05),说明造模成功;与阴性对照组比,鱼腥草醇提取物各组肛温、血清T3、T4、FT3、FT4值及肾上腺和甲状腺脏器质量均显著降低(均P0.05);体重、血清TSH值显著升高(P0.05)。结论鱼腥草醇提取物具有有效改善甲状腺素诱导小鼠甲亢的作用,为进一步揭示其传统功效的作用机理积累了工作基础。

Effect of alcohol extract of Houttuynia cordata Thunb on physiological indexes of hyperthyroidism mice induced by thyroid tablets

Objective To study the effect of Houttuynia cordata Thunb extract on hyperthyroidism mice induced by thyroid tablets . Methods Houttuynia cordata Thunb was used as raw material for extraction by alcohol. On the basis of establishing hyperthyroidism model mice by gavage with thyroid tablets, 40 male mice in the model group were randomly divided into negative control group (NM) and low, medium and high dose groups of Houttuynia cordata Thunb extract (R1, R2, R3). Another 10 male mice were taken as the normal control group (NC) and all the groups were administered once a day for 30 days. Results Compared with the normal group, there were significant differences in body weight, feeding, drinking water, rectal temperature, T3, T4, FT3, FT4,TSH values in the model group (P<0.05), indicating that the nodel was successful. Compared with the negative control group, rectal temperature, serum T3, T4, FT3, FT4 values and adrenal and thyroid organ mass were significantly decreased (P<0.05), weight and serum TSH values increased significandy (P<0.05) in drugs treated mice. Conclusion The alcohol extract of Houttuynia cordata Thunb can effectively improve thyroxine induced hyperthyroidism in mice. It provides a working basis for further revealing the scientific connotation and mechanism of its traditional efficacy.