| 基于实时荧光PCR对肉制品中羊肉的精确定量 |
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| 引用本文： | 金鹭,陈传君,林华,胡滨,韩国全,陈世界,张婧,安微,杨苗. 基于实时荧光PCR对肉制品中羊肉的精确定量[J]. 食品与发酵工业, 2020, 0(8): 246-253 |
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| 作者姓名： | 金鹭 陈传君 林华 胡滨 韩国全 陈世界 张婧 安微 杨苗 |
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| 作者单位： | 四川农业大学食品学院;成都海关;食品安全检测四川省重点实验室 |
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| 基金项目： | 国家海关总署科技计划项目(动物源性成分定量检测技术及在非法添加打假执法中的应用研究(2017IK019))。 |
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| 摘 要： | 该研究将实时荧光定量PCR技术(real-time fluorescence quantitative PCR,r PCR)与微滴式数字PCR技术(micro-droplet digital PCR,dd PCR)相结合,利用dd PCR建立拷贝数和羊肉质量的函数关系,确立了基于r PCR定量检测羊肉含量的方法。特异性实验结果表明,除绵羊和山羊外,非目标物种DNA未出现特异性扩增;灵敏度实验结果表明,该方法的最低检出限为0. 01 ng/μL;通过对已知成分的混合样品和市售样品的检测表明,该方法能够对含量在5%以上的羊肉进行准确定量。因此,该方法在肉制品中羊肉含量检测和掺假鉴别方面具有较好应用潜力。
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| 关 键 词： | 羊肉 实时荧光定量PCR 微滴数字PCR 定量检测 |
Accurate quantification of mutton in meat products by real-time quantitative PCR |
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| JIN Lu,CHEN Chuanjun,LIN Hua,HU Bin,HAN Guoquan,CHEN Shijie,ZHANG Jing,AN Wei,YANG Miao. Accurate quantification of mutton in meat products by real-time quantitative PCR[J]. Food and Fermentation Industries, 2020, 0(8): 246-253 |
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| Authors: | JIN Lu CHEN Chuanjun LIN Hua HU Bin HAN Guoquan CHEN Shijie ZHANG Jing AN Wei YANG Miao |
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| Affiliation: | (College of Food,Sichuan Agriculture University,Ya′an 625000,China;Chengdu Customs,Chengdu 61000,China;Food Safety Detection Key Laboratory of Sichuan,Chengdu 61000,China) |
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| Abstract: | In order to accurately quantify the content of mutton in meat products,this study established the functional relationship between copy number and mutton weight by using micro-droplet digital PCR(ddPCR).And a quantitative detection method of mutton content based on real-time fluorescence quantitative PCR(rPCR)were also developed.The result of specific experiment showed that the DNA of sheep and goat could be specifically amplified and the detection limit was 0.01 ng/μL.For the mixed samples and the commercial samples,this method could precisely quantify more than 5%of mutton contents in meat products.This method has a potential application in the detection of mutton content in meat products. |
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| Keywords: | mutton real-time fluorescence quantitative PCR droplet digital PCR quantitative determination |
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