唾液乳杆菌CCFM 1054通过改变肠道菌群缓解空肠弯曲杆菌在小鼠体内的感染

考察了唾液乳杆菌(Lactobacillus salivarius)CCFM 1054体外培养产酸及发酵上清液抑制空肠弯曲杆菌(Campylobacter jejuni)生长能力、对人工模拟胃肠液中的耐受、对共培养条件下的抑菌能力、对HT-29细胞的粘附以及自我形成生物膜的能力,并以鼠李糖乳杆菌LGG和植物乳杆菌N49作为对比菌株,分别干预空肠弯曲杆菌和弓形虫复合感染的小鼠。结果显示,CCFM 1054能显著改变小鼠肠道菌群的组成,降低空肠弯曲杆菌在小鼠体内的定植率并缓解其感染。肠道菌群变化和乳酸菌拮抗空肠弯曲杆菌相关的体内外特性的相关性分析表明,CCFM 1054对细胞的高粘附性及其较强的生物膜形成能力使得其能在小鼠体内显著改变肠道菌群丰度。

Lactobacillus salivarius CCFM 1054 alleviates the infection of Campylobacter jejuni in mice by regulating the gut microbiota

In this study,the biological characteristics of Lactobacillus salivarius CCFM 1054 in vitro were evaluated,such as the ability of acid production,the antibacterial ability of free supernatant for Campylobacter jejuni growth,the tolerance to artificial gastrointestinal fluids,the inhibition to co-cultured C.jejuni,the adhesion to HT-29 cell and the ability of biofilms generation.It was founded that CCFM 1054 had strong acid-producing ability and could inhibit the growth of C.jejuni in vitro.At the same time,it had good tolerance under artificial simulated gastrointestinal fluid,high adhesion to HT-29 cells and strong self-film-forming ability.The L.rhamnosus LGG and L.plantarum N49 were used as control strains to CCFM 1054 when administered to C.jejuni and Toxoplasma co-infected mice by gavage.The results showed that CCFM 1054 could significantly change the composition of gut microbiota,such as Firmicutes,Bacteroidetes and Proteobacteria at the phylum level and Campylobacter,Lactobacillus,Pediococcus,Faecoccus,Coprococcus and Unclassified Enterobacteriaceae at the genera level.Changes in gut microbes reduced the colonization of C.jejuni in mice and alleviated the infection of C.jejuni in vivo.The correlation between the changes of gut microbiota and the biological characteristics of lactic acid bacteria in vitro indicated that CCFM 1054's high adhesion to HT-29 cell and strong biofilm-forming ability made it change the gut microbiota in mice significantly.