柠檬马鞭草精油结合亚麻籽胶对大菱鲆肌原纤维蛋白生化特性及构象的影响

本文旨在探究柠檬马鞭草精油结合亚麻籽胶对冰温贮藏大菱鲆肌原纤维蛋白(myofibril protein,MP)生化特性及构象的影响。以未涂膜处理组为对照,实验组用添加质量浓度为0%、0.128%、0.64%和1.28%柠檬马鞭草精油的复合涂膜处理,分析冰温贮藏期间大菱鲆MP结构及生化特性的变化。结果表明:随着贮藏时间的延长,所有组大菱鲆MP的总巯基含量和Ca²⁺-ATPase活性均不断下降,游离氨基酸含量不断上升,且经0.128%质量浓度柠檬马鞭草精油复合涂膜处理的大菱鲆MP的总巯基含量在贮藏15 d后比对照组增加了22.04%,表明复合涂膜显著抑制了MP的氧化。傅里叶变换红外光谱测定发现复合涂膜处理抑制了α-螺旋相对含量的减少,同时涂膜能显著减缓内源荧光强度的下降速率,维持大菱鲆MP结构的稳定。此外,透射电镜观察表明添加0.128%质量浓度柠檬马鞭草精油复合涂膜处理的大菱鲆在贮藏后期依然保持较完整的肌原纤维结构。因此,柠檬马鞭草精油-亚麻籽胶复合涂膜能显著抑制冰温贮藏大菱鲆MP的氧化和降解,且精油质量浓度为0.128%时抑制效果最佳,表明这种复合涂膜用来保鲜大菱...

Effects of verbena oil combined with flaxseed gum on the structure and biochemical characteristics of myofibrillar protein in turbot

This study aims to investigate the effect of verbena oil combined with flaxseed gum on the structure and biochemical characteristics of myofibrillar protein(MP)in turbot during super chilling storage.The experimental groups were treated with composite coatings of concentration of 0%,0.128%,0.64% and 1.28% verbena oil and the control group was treated with water.The changes in MP structure and biochemical characteristics were examined during super chilling storage.The results showed that as the storage time increased,the total sulfhydryl content and Ca^2+-ATPase activity of turbot MP in all groups decreased continuously and free amino acid content showed upward trend continuously.In addition,the total sulfhydryl content of turbot MP treated with 0.128% verbena oil composite coating increased by 22.04% compared to that of the control group after 15 days,indicating that the composite coatings significantly inhibited the oxidation of MP.Fourier transform infrared spectroscopy(FT-IR)measurement showed that the composite coating inhibited the decrease of the relative content of α-helix,and the coating slowed down the rate of decline of intrinsic fluorescence as well.Thus,the structure of turbot MP remained stable.Furthermore,transmission electron micrographs indicated that turbot treated with composite coating containing 0.128% verbena oil kept the relatively intact myofibrillar structure in the later storage period.Therefore,verbena oil combined with flaxseed gum can significantly inhibit the oxidation and degradation of the turbot MP during super chilling storage,and the inhibitory effect is the best when the concentration of the oil is 0.128%,which indicate that the composite coating is a promising method to preserve turbot.