| PLGA/TSF静电纺纳米纤维的仿生矿化及生物相容性 |
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| 引用本文： | 王倩,邵伟力,高艳菲,何建新,陈利,崔世忠.PLGA/TSF静电纺纳米纤维的仿生矿化及生物相容性[J].材料科学与工程学报,2017,35(2). |
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| 作者姓名： | 王倩 邵伟力 高艳菲 何建新 陈利 崔世忠 |
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| 作者单位： | 1. 中原工学院纺织学院,河南郑州450007;纺织服装产业河南省协同创新中心,河南郑州450000;2. 中原工学院纺织学院,河南郑州450007;天津工业大学先进纺织复合材料教育部重点实验室,天津300387;纺织服装产业河南省协同创新中心,河南郑州450000;3. 中原工学院纺织学院,河南郑州,450007;4. 天津工业大学先进纺织复合材料教育部重点实验室,天津,300387 |
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| 基金项目： | 国家自然科学基金资助项目，NSFC-河南人才培养联合基金资助项目，河南省教育厅科学技术研究重点资助项目，郑州市普通科技攻关资助项目 |
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| 摘 要： | 利用静电纺丝和模拟体液仿生矿化技术制备了聚乳酸-羟基乙酸共聚物/柞蚕丝素/羟基磷灰石((PLGA/TSF/HA)骨组织工程复合支架。通过扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射测试(XRD)和热重分析(TG)对复合纳米纤维的形貌结构进行了表征。此外,在复合纳米纤维支架材料上接种人骨髓间充质干细胞(hMSCs),通过四甲基偶氮噻唑蓝比色(Four methyl azo thiazole blue colorimetric,MTT)法,观察细胞在材料表面的生长情况评价纳米纤维的生物相容性。结果显示,PLGA/TSF纳米纤维毡具有精细的三维结构,纤维直径分布均匀,表面光滑。矿化后HA颗粒均匀地分布在PLGA/TSF纳米纤维表面,矿物含量约占63%。与PLGA/TSF纳米纤维支架相比,PLGA/TSF/HA纳米纤维支架的亲水性、生物相容性都得到显著提高。
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| 关 键 词： | 聚乳酸-羟基乙酸共聚物 柞蚕丝素 羟基磷灰石 生物相容性 |
Biomimetic Mineralization and Biocompatibility of PLGA/TSF Electrospun Nanofibers |
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| Abstract: | PLGA/TSF/HA electrospun nanofibers for bone tissue engineer composite scaffolds were prepared by technologies of electrospinning and simulated body fluid biomimetic mineralization.The morphology and structure of composite nanofibers were characterized by SEM,TEM,XRD and TG.Besides,hMSCs were cultured in vitro to study the biocompatible properties of composite nanofibers,which were characterized by Four methyl azo thiazole blue colorimetric (MTT) and SEM.The results indicated that PLGA/TSF nanofibers have the refined three-dimensional structure and the uniform diameter distribution with the smooth surface.HA particles uniformly distributed on the surface of PLGA/TSF nanofibers after mineralization,and the content of mineral was about 63％.The hydrophily and biocompatibility of PLGA/TSF/HA nanofiber scaffolds were significantly enhanced compared with PLGA/TSF. |
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| Keywords: | poly(lactic-co-glycolic acid) (PLGA) tussah silk fibroin (TSF) hydroxyapatite (HA) Biocompatible properties |
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