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| 实时荧光定量PCR技术检测转基因大豆方法的建立 | |
| 引用本文： | 陈颖,徐宝梁,苏宁,王媛,王丙武,葛毅强. 实时荧光定量PCR技术检测转基因大豆方法的建立[J]. 食品与发酵工业, 2003, 29(8): 65-69 |
| 作者姓名： | 陈颖 徐宝梁 苏宁 王媛 王丙武 葛毅强 |
| 作者单位： | 1. 中国进出口商品检验技术研究所,北京,100025 2. 中国农业大学食品学院,北京,100049 3. 科技部中国农村技术开发中心,北京,100045 |
| 基金项目： | 科技部社会公益研究专项资金项目 |
| 摘 要： | 采用实时荧光定量PCR技术 ,通过使用特异的引物和探针 ,对大豆中的内源基因Lectin和转基因大豆中的外源基因EPSPS进行了定量检测 ,建立了Monsanto公司生产的商业化转基因大豆Roundup Ready○R的定量PCR检测方法。该方法的检测灵敏度 <0 0 1% ,是国际上设定的转基因最低限量的 10 0倍 |
| 关 键 词： | 荧光定量PCR 转基因大豆 转基因检测 |
| 修稿时间： | 2003-04-14 |
Real-time Quantitative Polymerase Chain Reaction Methods for Genetically Modified Soybean |
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| Chen Ying Xu Baoliang Su Ning Wang Yuan Wang Bingwu Ge Yiqiang. Real-time Quantitative Polymerase Chain Reaction Methods for Genetically Modified Soybean[J]. Food and Fermentation Industries, 2003, 29(8): 65-69 | |
| Authors: | Chen Ying Xu Baoliang Su Ning Wang Yuan Wang Bingwu Ge Yiqiang |
| Affiliation: | Chen Ying 1 Xu Baoliang 1 Su Ning 1 Wang Yuan 2 Wang Bingwu 1 Ge Yiqiang 31 |
| Abstract: | A quantitative method was developed to detect transgenic "Roundup Ready" soybean (Monsanto) by real time quantitative PCR Special primers and probes were used to amplify the endogenous gene lectin and exogenous gene CP4EPSPS The detection limit of this method was 0 01% which was 100 times lower than the lowest international labeling threshold The statistical analysis showed that the accuracy and precision of this method was in agreement with other quantitative GMO detection systems |
| Keywords: | real time quantitative PCR, " Roundup Ready" soybean, GMO detection, |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |