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| 桅子黄多酚含量与抗氧化活性的相关性研究 | |
| 引用本文： | 陈阳,张浩,王文君,向灿辉,何晓彤,蔡乐.桅子黄多酚含量与抗氧化活性的相关性研究[J].食品工业科技,2011(10):125-128. |
| 作者姓名： | 陈阳 张浩 王文君 向灿辉 何晓彤 蔡乐 |
| 作者单位： | 1. 遵义医学院珠海校区化学教研室,广东珠海,519041 2. 四川大学华西药学院生药学教研室,四川成都,610041 3. 云南大学化学科学与工程学院 教育部自然资源药物化学重点实验室,云南昆明,650091 |
| 基金项目： | 贵州省高层次人才特助基金(2010); 贵州省国际合作项目基金(2009GZ52794) |
| 摘 要： | 目的：揭示栀子黄抗氧化能力是否与多酚含量有关，为栀子黄中抗氧化能力成分的确定提供线索。方法：分别采用还原能力实验、DPPH自由基清除能力实验和亚铁离子螯合实验测定西红花苷、绿原酸、栀子粗提物和栀子黄的抗氧化活性，同时分别采用HPLC和UV来测定栀子粗提物和栀子黄中西红花苷和多酚的含量。结果：在还原能力和DPPH自由基清除活性模型中，栀子黄色素中起抗氧化作用的主要化学成分是多酚类，而不是西红花苷。另一方面．与前两个模型不同，西红花苷和多酚类成分都具有明显的亚铁离子螯合活性，栀子黄中具有螯合活性的主要化学成分同时包括西红花苷和多酚类。结论：结合前期研究结果推测，栀子黄抗氧化作用的主要化学成分是多酚类．而西红花苷具有明显的亚铁离子螯合活性。 |
| 关 键 词： | 栀子黄 西红花苷 多酚 抗氧化 UV HPLC |
Study on relationship between polyphenols content and antioxidant effect of gardenia yellow |
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| CHEN Yang,ZHANG Hao,WANG Wen-jun,XIANG Can-hui,HE Xiao-tong,CAI Le.Study on relationship between polyphenols content and antioxidant effect of gardenia yellow[J].Science and Technology of Food Industry,2011(10):125-128. | |
| Authors: | CHEN Yang ZHANG Hao WANG Wen-jun XIANG Can-hui HE Xiao-tong CAI Le |
| Affiliation: | CHEN Yang1,ZHANG Hao2,WANG Wen-jun1,XIANG Can-hui1,HE Xiao-tong1,CAI Le3,(1. Department of Chemistry,Zunyi Medical College Zhuhai Campus,Zhuhai 519041,China,2. West China School of Pharmacy,Sichuan University,Chengdu 610041,3. Key Laboratory of Medicinal Chemistry for Nature Resource,Ministry of Education,School of Chemical Science and Technology,Yunnan University,Kunming 650091,China) |
| Abstract: | Objective:In order to investigate the relationship between polyphenols content and antioxidant effect of gardenia yellow. Methods:Reducing power assay,DPPH radical scavenging effect and chelating ability on ferrous ions were determined. Then,with the aid of HPLC and UV,crocins and polyphenols content were measured. Results:In terms of reducing power assay and DPPH radical scavenging assay,only polyphenols content showed relationship with antioxidant effect of gardenia yellow. In addition,crocins and polyphe... |
| Keywords: | gardenia yellow crocins polyphenols antioxidant UV HPLC |
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