| 产木聚糖酶和纤维素酶真菌的酶学性质分析 |
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| 引用本文： | 麦国琴,许晓萍,余翠媚,张立国,王娟. 产木聚糖酶和纤维素酶真菌的酶学性质分析[J]. 食品研究与开发, 2011, 32(9): 179-183 |
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| 作者姓名： | 麦国琴 许晓萍 余翠媚 张立国 王娟 |
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| 作者单位： | 深圳大学生命科学学院,广东深圳,518060 |
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| 基金项目： | 深圳市南山区科技计划项目(南科院0031); 深圳大学专题研究型教改项目(JG2010059);深圳大学青年教师教改项目(JG2010111) |
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| 摘 要： | 红树林土壤微生物资源丰富,从深圳福田红树林土壤中分离了80株真菌,经过产酶发酵复筛,获得2株同时具有较高木聚糖酶和纤维素酶活的真菌。通过菌株形态观察和分子鉴定,确定菌株1为草酸青霉(Penicillium oxalicum),菌株2为曲霉属的Aspergillus piperis。菌株1的木聚糖酶和纤维素酶酶活分别为42.16 IU和3.05 IU,菌株2的木聚糖酶和纤维素酶酶活分别为39.01 IU和0.87 IU。同时,对其进行酶学性质研究表明,2菌株木聚糖酶的最适温度均为50℃;菌株1纤维素酶的最适温度是60℃,菌株2纤维素酶的最适温度是70℃,具有一定的耐热性。两菌株纤维素酶的最适pH均为3.0;菌株1木聚糖酶的最适pH为5.0,菌株2木聚糖酶的最适pH为4.0。
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| 关 键 词： | 纤维素酶 木聚糖酶 土壤真菌 青霉 曲霉 |
Screening of Cellulase and Xylanase-pruducing Fungi from Mangrove Soil and Characterization of the Enzymatic Reactions |
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| MAI Guo-qin,XU Xiao-ping,YU Cui-mei,ZHANG Li-guo,WANG Juan. Screening of Cellulase and Xylanase-pruducing Fungi from Mangrove Soil and Characterization of the Enzymatic Reactions[J]. Food Research and Developent, 2011, 32(9): 179-183 |
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| Authors: | MAI Guo-qin XU Xiao-ping YU Cui-mei ZHANG Li-guo WANG Juan |
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| Affiliation: | MAI Guo-qin,XU Xiao-ping,YU Cui-mei,ZHANG Li-guo,WANG Juan (College of life sciences Shenzhen university,Shenzhen 518060,Guangdong,China) |
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| Abstract: | Mangrove ecological system has rich resources of microorganisms in the soil.Eighty strains of funfi were isolated from mangrove environment.Two strains of cellulase and xylanase-pruducing fungi were obtained after rescreening fermentation for enzyme production.The enzyme activities of cellulase and xylanase of strain No.1 is 42.16 IUand 3.05 IU respectively.The enzyme activities of cellulase and xylanase of strain No.2 is 39.01IU and 0.87 IU respectively.The optimum temperature of xylanase of two strains ar... |
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| Keywords: | cellulase xylanase soil fungi Penicillium Aspergillus |
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