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| 一种检测肉品的新型通用单引物多重PCR技术 | |
| 引用本文： | 商颖,许文涛,元延芳,梁志宏,石慧,翟志芳,张雅楠,罗云波,黄昆仑. 一种检测肉品的新型通用单引物多重PCR技术[J]. 食品工业科技, 2011, 0(9): 416-419 |
| 作者姓名： | 商颖 许文涛 元延芳 梁志宏 石慧 翟志芳 张雅楠 罗云波 黄昆仑 |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083 2. 中国农业大学食品科学与营养工程学院,北京100083／农业部转基因生物食用安全监督检验测试中心（北京）,北京100083 3. 农业部转基因生物食用安全监督检验测试中心（北京）,北京,100083 |
| 基金项目： | 国家高技术研究发展计划(863)项目(2006AA10Z440); 国家自然基金(30800770); 转基因生物重大专项(2008ZX08012-001) |
| 摘 要： | 在市场中,牛、羊肉中掺杂价格便宜的猪肉,以次充好的欺骗行为屡见不鲜,而且近些年来出现疯牛病、禽流感等疾病,肉品的种属鉴定变得至关重要。随着分子生物学的深入研究,多重PCR技术得到了飞快的发展,但是其扩增效率和检测限达不到理想要求。为寻找快速、灵敏、特异的检测生肉品种的方法,在普通多重PCR的基础上发展了一种新的通用单引物多重PCR（CSP-M-PCR）技术,该方法的体系中,所有目的片段共用一样的上游引物进行扩增,检测限可达5μmol/L。此方法不仅弥补了普通多重PCR扩增效率较低、检测限不理想的缺点,还提高了检测的灵敏度、降低了检测的成本。 |
| 关 键 词： | 多重PCR 通用单引物 同时检测 肉品种属 |
Novel common single primer multiplex PCR for detecting various meat species simultaneously |
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| SHANG Ying,XU Wen-tao,,,YUAN Yan-fang,LIANG Zhi-hong,SHI Hui,ZHAI Zhi-fang,ZHANG Ya-nan,LUO Yun-bo,HUANG Kun-lun. Novel common single primer multiplex PCR for detecting various meat species simultaneously[J]. Science and Technology of Food Industry, 2011, 0(9): 416-419 | |
| Authors: | SHANG Ying XU Wen-tao YUAN Yan-fang LIANG Zhi-hong SHI Hui ZHAI Zhi-fang ZHANG Ya-nan LUO Yun-bo HUANG Kun-lun |
| Affiliation: | SHANG Ying1,XU Wen-tao1,2,*,YUAN Yan-fang2,LIANG Zhi-hong2,SHI Hui1,ZHAI Zhi-fang1,ZHANG Ya-nan2,LUO Yun-bo1,HUANG Kun-lun1,*(1. College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083,China,2. The Supervision,Inspection & Testing Center of Genetically Modified Food Safety,Ministry of Agriculture,China) |
| Abstract: | Nowadays in the market,add cheap pork into beef or mutton,sell seconds at best quality prices always happen.In recent years,since the emergence of some diseases,such as mad cow disease,avian flu and so on,meat species identification becomes more and more critical.With in-depth study of molecular biology,multiplex PCR technology has made a rapid development,however,the low amplification efficiency and detection limit don't live up to the satisfactory requirements.In order to find a quick,sensitive and specif... |
| Keywords: | multiplex PCR common single primer simultaneous detection meat species |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |