| HPLC分离测定红心薯中类胡萝卜素 |
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| 引用本文： | 吉宏武 施兆鹏. HPLC分离测定红心薯中类胡萝卜素[J]. 食品与生物技术学报, 2001, 20(3): 299-301 |
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| 作者姓名： | 吉宏武 施兆鹏 |
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| 作者单位： | 1. 无锡轻工大学食品学院,
2. 湖南农业大学茶学系, |
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| 摘 要： | 应用高效液相色谱法分离和测定红心薯中类胡萝卜素.样品制备采用已烷-丙酮-乙腈-甲苯混合溶剂与40%氢氧化钾甲醇溶液浸提、皂化的方法，使用Nova-PakC18不锈钢柱,乙腈-三氯甲烷-叔丁醇作三元流动相,流量线性梯度洗脱等色谱条件,在440nm波长下检测,红心薯中主要类胡萝卜素得到了较好的分离.以外标定量法对红心薯中的4种主要类胡萝卜素(β-胡萝卜素,叶黄质,堇黄质,新黄质)进行了定量，结果较为满意.
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| 关 键 词： | 红心薯；类胡萝卜素；高效液相色谱；分离测定 |
| 文章编号： | 1009-038X(2001)03-0299-03 |
| 修稿时间： | 2000-10-08 |
HPLC Separation and Determination of Carotenoids in Ipomoea batatas |
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| JI Hong wu,SHI Zhao peng. HPLC Separation and Determination of Carotenoids in Ipomoea batatas[J]. Journal of Food Science and Biotechnology, 2001, 20(3): 299-301 |
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| Authors: | JI Hong wu SHI Zhao peng |
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| Affiliation: | JI Hong wu 1,SHI Zhao peng 2 |
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| Abstract: | The carotenoids from Ipomoea batatas were separated and determined by HPLC. Results show that the main carotenoids in Ipomoea batatas can be successfully separated in 10min with detection at 440nm by extracting the sample in hexane -acetone-ethanol-toluene and 40% potassium hydroxide methanol for twelve hours ,employing Nova-Pak C18 stainliss steel column (3.9×150 mm),using acetonitrile -chloroform-methyl carbinol (83∶15∶2)as flow phase, applying linear gradient eluting with the rate of flow, with the temperature of column at 25 ℃.Four kinds of main carotenoids from Ipomoea batatas were determined by external standards. |
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| Keywords: | Ipomoea batatas carotenoids HPLC separation and determination |
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