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The inactivation of the non-enveloped enterovirus 71 (EV71) by a novel disinfectant gel formulation for topical use |
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| Authors: | Hai-Qiu Ma Ying Wang Yong-Hong Mao Shu-Yan Wang Yu-Pu Zhang Chen-Qiang Zuo |
| Affiliation: | 1. Tianjin International Joint Academy of Biomedicine, The Tianjin Economic-Technological Development Area (TEDA), Tianjin, China;2. Xu He (Tianjin) Medical Technology Co., Ltd., The Tianjin Economic-Technological Development Area (TEDA), Tianjin, China |
| Abstract: | Background: Hand-foot-mouth disease may cause severe central nervous system complications and even death, that is induced mainly by enterovirus 71 (EV71), which is a non-enveloped virus. Inactivation of the EV71 on hands could effectively inhibit the transmission. However, the inactivations of the EV71 by conventional disinfectants including the alcohols are poor, due to the high stability of the EV71. A novel pyridyl imidazolidinone compound (TJAB1099) was designed to specifically inhibit EV71 replication in vitro. It may potentially be developed as formulations applied on hands for EV71 transmission control. Methods: The stress stability of TJAB1099 was first evaluated after storing in high temperature (60?°C, RH 10%), high humidity (25?°C, RH90%), and the high-intensity photolysis (4500?Lx ± 500?Lx) for 15 days, respectively. A wash-free antimicrobial gel containing the TJAB1099 was developed using the copolymer carrier. The antiviral activity, the acute oral toxicity, and the local irritation of the antimicrobial gel were evaluated accordingly. Results: The results indicated that the TJAB1099 was stable during the storage in high temperature and humidity. However, a significant change (p?p?p? Conclusions: The results suggest that the antimicrobial gel containing TJAB1099 was safe and could effectively inhibit EV71 transmission in vitro. |
| Keywords: | Hand-foot-and-mouth disease enterovirus 71 antimicrobial gel copolymer formulation |