| 磺胺二甲嘧啶完全抗原的制备及鉴定 |
| |
| 引用本文： | 王颖,姚笛,王月,安宇,张东杰,柳增善.磺胺二甲嘧啶完全抗原的制备及鉴定[J].粉末涂料与涂装,2011,24(4). |
| |
| 作者姓名： | 王颖 姚笛 王月 安宇 张东杰 柳增善 |
| |
| 作者单位： | 王颖,WANG Ying(黑龙江八一农垦大学食品学院食品科学教研室,黑龙江大庆163319;吉林大学教育部人兽共患病重点实验室细菌室,长春130062);姚笛,王月,张东杰,YAO Di,WANG Yue,ZHANG Dong-jie(黑龙江八一农垦大学食品学院食品科学教研室,黑龙江大庆,163319);安宇,AN Yu(黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319);柳增善,LIU Zeng-shan(吉林大学教育部人兽共患病重点实验室细菌室,长春,130062) |
| |
| 摘 要： | 目的制备磺胺二甲嘧啶(Sulfadimidine,SM2)完全抗原,并进行鉴定。方法采用重氮化偶联法制备SM2免疫抗原(SM2-BSA)和检测抗原(SM2-OVA),定性和定量分析偶联后的完全抗原,并制备SM2单抗。结果经1%琼脂糖凝胶电泳和10%SDS-PAGE分析表明,完全抗原SM2-BSA和SM2-OVA偶联成功;SM2与载体蛋白BSA和OVA的分子结合比分别为23∶1和17∶1;SM2-BSA和SM2-OVA的蛋白浓度分别为3.24和2.29 mg/ml;完全抗原SM2-BSA能刺激小鼠产生高效、特异的抗SM2抗体;SM2单抗与OVA和BSA无交叉反应。结论已成功制备了完全抗原SM2-BSA和SM2-OVA,为下一步建立灵敏度更高、特异性更强、操作更简便的免疫学检测方法奠定了基础。
|
| 关 键 词： | 磺胺二甲嘧啶 完全抗原 分子结合比 交叉反应 |
Preparation and Identification of Complete Sulfadimidine Antigen |
| |
| WANG Ying,YAO Di,WANG Yue,AN Yu,ZHANG Dong-jie,LIU Zeng-shan.Preparation and Identification of Complete Sulfadimidine Antigen[J].Chinese Journal of Biologicals,2011,24(4). |
| |
| Authors: | WANG Ying YAO Di WANG Yue AN Yu ZHANG Dong-jie LIU Zeng-shan |
| |
| Abstract: | Objective To prepare and identify complete sulfadimidine(SM2) antigen.Methods SM2-BSA as antigen for immunization and SM2-OVA as antigen for detection were prepared by coupling with diazotization,and analyzed qualitatively and quantitatively.Results Both 1% agarose gel electrophoresis and 10% SDS-PAGE showed that complete antigens SM2-BSA and SM2-OVA were coupled successfully.The molecule conjugate ratios of SM2 to BSA and to OVA were 23 ∶ 1 and 17 ∶ 1 respectively.The protein concentrations of SM2-BSA and SM2-OVA were 3.24 and 2.29 mg/ml respectively.Complete SM2-BSA antigen stimulated high titer specific antibody against SM2 in mice.The monoclonal antibody against SM2 showed no cross reaction with OVA or BSA.Conclusion Complete SM2-BSA and SM2-OVA antigens were successfully prepared,which laid a foundation of further developing more sensitive,specific amd simple method for immunological test. |
| |
| Keywords: | Sulfadimidine(SM2) Complete antigen Molecule conjugate ratio Cross reaction |
| 本文献已被 万方数据 等数据库收录！ |
|
