三七总皂甙对大鼠肺动脉血管平滑肌细胞增殖和凋亡的影响及其机制

目的探讨三七总皂甙(Panax notoginseng saponins,PNS)对大鼠肺动脉血管平滑肌细胞(Pulmonary artery smoothmuscle cell,PASMC)增殖和凋亡的影响及其机制。方法将PASMC分为5组:对照组(不给药)、bFGF组(8 ku/L)、bFGF+低、中、高剂量PNS组(bFGF 8 ku/L+300、450、600μg/L PNS),给药24 h后,采用MTT法检测PASMC的增殖活力;流式细胞仪检测细胞周期分布和细胞凋亡率;Westernblot检测细胞中胱天蛋白酶-3(caspase-3)蛋白的表达。结果 PNS干预可抑制PASMC增殖,阻遏bFGF诱导的PASMC进入S期,提高滞留在G0/G1期细胞的比例,并使PASMC凋亡率和caspase-3蛋白表达上调,PASMC凋亡率达(18.70%±0.33%)～(28.40%±0.79%),是bFGF诱导组的10～14倍。结论 PNS能显著抑制bFGF诱导的PASMC增殖,促进其凋亡,其机制可能与其调控细胞周期转换、激活caspase-3蛋白的表达有关。

Effect of Total Panax Notoginseng Saponins on Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells in Rats and Relevant Mechanism

Objective To investigate the effect of total panax notoginseng saponins(PNS) on proliferation and apoptosis of pulmonary artery smooth muscle cells(PASMCs) in rats as well as the relevant mechanism.Methods PASMCs were divided into control(untreated),bFGF(8 ku/L) as well as bFGF + PNS at low(300 μg/L),moderate(450 μg/L) and high(600 μg/L) dosage groups,and determined for proliferation activity by MTT method,for distribution of cell cycle and apoptosis rate by flow cytometry and for expression of caspase-3 by Western blot 24 h later.Results PNS intervention inhibited the proliferation of PASMCs,arrested the cell cycle of PASMCs induced by bFGF into S stage,and increased the proporation of PASMCs at G0/G1 stages,the apoptosis rate of PASMCs as well as the expression level of caspase-3.The apoptosis rate of PASMCs treated with PNS was(18.70% ± 0.33%) ~(28.40% ± 0.79%),which was 10 ~ 14 times higher than those treated with bFGF.Conclusion PNS significantly inhibited the bFGF-induced proliferation and promoted the apoptosis of PASMCs by a potential mechanism which might be associated with regulating the conversion of cell cycle and activating the caspase-3 expression.