Fluorescent dye encapsulated ZnO particles with cell-specific toxicity for potential use in biomedical applications |
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Authors: | Hua Wang Denise Wingett Mark H Engelhard Kevin Feris K M Reddy Paul Turner Janet Layne Cory Hanley Jason Bell Dmitri Tenne Chongmin Wang Alex Punnoose |
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Affiliation: | (1) Department of Physics, Boise State University, Boise, ID 83725, USA;(2) Department of Biological Sciences, Boise State University, Boise, ID 83725, USA;(3) Mountain States Tumor and Medical Research Institute, Boise, ID 83702, USA;(4) Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA;(5) Department of Physics, Indian Institute of Science, Bangalore, 560012, India |
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Abstract: | Fluorescein isothiocyanate (FITC)-encapsulated SiO2 core-shell particles with a nanoscale ZnO finishing layer have been synthesized for the first time as multifunctional “smart”
nanostructures. Detailed characterization studies confirmed the formation of an outer ZnO layer on the SiO2–FITC core. These ~200 nm sized particles showed promise toward cell imaging and cellular uptake studies using the bacterium
Escherichia coli and Jurkat cancer cells, respectively. The FITC encapsulated ZnO particles demonstrated excellent selectivity in preferentially
killing Jurkat cancer cells with minimal toxicity to normal primary immune cells (18% and 75% viability remaining, respectively,
after exposure to 60 μg/ml) and inhibited the growth of both gram-positive and gram-negative bacteria at concentrations ≥250–500 μg/ml
(for Staphylococcus aureus and Escherichia coli, respectively). These results indicate that the novel FITC encapsulated multifunctional particles with nanoscale ZnO surface
layer can be used as smart nanostructures for particle tracking, cell imaging, antibacterial treatments and cancer therapy. |
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