Polymeric protein formation during pasta-making with barley and semolina mixtures,and prediction of its effect on cooking behaviour and acceptability |
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Authors: | C. Lamacchia A. Baiano S. Lamparelli C. Terracone A. Trani A. Di Luccia |
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Affiliation: | 1. Dipartimento di Scienze degli Alimenti, Università degli Studi di Foggia, Via Napoli, 25 – 71100 Foggia, Italy;2. Istituto per la Ricerca e le Applicazioni Biotecnologiche per la Sicurezza e la Valorizzazione dei Prodotti Tipici e di Qualità, Università di Foggia, Via Napoli, 25 – 71100 Foggia, Italy;3. Dipartimento di Biologia e Chimica Agro-Forestale ed Ambientale, Università di Bari, Via Amendola, 165/A – 70126 Bari, Italy |
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Abstract: | Spaghetti were produced in a pilot plant from semolina and semolina blended with increasing amounts of barley flour. According to size exclusion high-performance liquid chromatography (SE-HPLC), barley proteins interact with semolina proteins during pasta making, forming polymers of high molecular weight. Of these, the unextractable polymeric proteins (UPP) were at significantly higher concentrations than in spaghetti made from semolina. The decrease of both S–S bonds and –SH free groups in barley semolina spaghetti, with respect to that made of semolina, suggested that polymerisation among the different classes of proteins involves a new bonding arrangement. Due to β-glucan hydrophilicity and competition with starch for water, the replacement of increasing amounts of semolina with barley flour was able to increase the optimal cooking time. The sensory properties of composite spaghetti were judged as better than the control because of the higher firmness and the lower bulkiness and stickiness of the former. |
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Keywords: | Acceptability Barley Protein size distribution Quality prediction Spaghetti cooking quality Unextractable polymeric proteins |
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